Smmc 7721

Human normal hepatocyte LO2 cell line and liver cancer cell line SMMC-7721 were purchased from Beijing Solarbio Science & Technology Co., Ltd. and they were incubated in DMEM medium (including 10% fetal bovine serum, 100 U/mL penicillin, 100 U/mL streptomycin) in cell culture incubator (37 °C, 5% CO₂).
LO2 and SMMC-7721 cells were inoculated with the density of (5 × 10³/well) in 96 well plates and incubated for 24 h. The cell culture media without drug were used as the control group, and the SRF solution, SRF-BSANPs, and FA-SRF-BSANPs were used as the experimental group. After the cells were adhered, the old medium was removed, and 0.2 mL of medium containing drug was added to each well (three SRF preparations were diluted to 60.0, 40.0, and 20.0 µg/mL with the medium, respectively.) and incubated for 24 h. Then 15.0 µL MTT solution (5 mg/mL) was added to each well in the dark. The medium was removed after 4 h, and the DMSO was added to dissolve formazan, followed by measurement of the absorbance at 490 nm (A) with DNM-9602A microplate reader (Beijing PERLONG medical company) to calculate the inhibition ratio.
$\text{Cell}\text{inhibition}\text{ratio}\left(\%\right)=1-A_{\text{experimental}\text{group}}/A_{\text{control}\text{group}}$

HepG2, MNK-45, MCF-7, SW620, and A549 cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). SMMC-7721 cell line was purchased from BeNa Culture Collection (Beijing, China). MNK-45, SMMC-7721, and SW620 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Solarbio, Beijing, China); andHepG2, MCF-7,and A549 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Solarbio, Beijing, China).All cells were incubated at 37 °C in a humidified 5% CO₂ atmosphere.