Three serogroups of Shiga toxin-producing E. coli were used in this study. E. coli O157:H7 H1730 was a human isolate from a lettuce outbreak and E. coli O157:H7 (ATCC 43895) was from a 1982 ground beef outbreak. Both isolates were obtained from the culture collection at the Center for Food Safety, University of Georgia. E. coli O121:H19 (strain TW08980) and E. coli O26:H11 (strain 3012-03) were from a 2016 Missouri flour outbreak and obtained from the Michigan State University STEC Center. Isolates were revived from frozen storage by transferring to Tryptic Soy Broth (TSB, Neogen, Lansing, MI, USA) and incubating at 37°C for 24 h. The bacterial strains were evaluated for antibiotic resistance to ampicillin and streptomycin by streaking on Tryptic Soy Agar (TSA; Neogen Lansing, MI, USA) amended with 100 μg/ml of ampicillin, 100 μg/ml of streptomycin, and a combination of 100 μg/ml streptomycin and ampicillin. Plates were placed at an incubation temperature of 37°C for 24 h and observed for colony formation. Cultures that did not yield colonies in the antibiotic amended plates were considered to be susceptible to the antibiotics (Table 1 and Figure 1 ).
Tryptic soy broth tsb
Manufactured by Neogen
Sourced in United States
Tryptic Soy Broth (TSB) is a general-purpose growth medium used for the cultivation of a wide range of microorganisms, including bacteria and fungi. It provides the necessary nutrients and growth factors to support the proliferation of these organisms in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Tryptic soy agar tsa, by Neogen (3 mentions)
Ethanol, by Chempur (1 mentions)
Chloroform, by Chempur (1 mentions)
Isopropanol, by Chempur (1 mentions)
Dichloromethane, by Chempur (1 mentions)
Anaeropack system, by Mitsubishi (1 mentions)
Hexane, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Cv solution, by Merck Group (1 mentions)
Tryptone, by Avantor (1 mentions)
Sodium phosphate dibasic, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Merck Group (1 mentions)
M9 minimal salts 5, by Merck Group (1 mentions)
Ferric ammonium citrate, by Merck Group (1 mentions)
6 protocols using tryptic soy broth tsb
1
Antibiotic Resistance Profiling of Shiga Toxin-Producing E. coli
2
Synthesis and Characterization of PCL Polymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PCL pellets (Mn 80,000, cat no.: 440744-500G) were obtained from Sigma Aldrich (Gillingham, U.K.), GS, USP grade was obtained from Fluorochem Ltd. (Glossop, U.K.). Solvents used such as dichloromethane (DCM), chloroform, ethanol, and iso-propanol, and components to prepare the phthaldialdehyde reagent were obtained from Chempur (Piekary Śląskie, Poland). Tryptic Soy Broth (TSB) and tryptic soy agar (TSA) were obtained from Neogen® (Lansing, MI, USA).
3
Cultivation of Clostridium perfringens ATCC 13,124
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A primary culture of C. perfringens ATCC 13,124 ™ was started by inoculating a fresh colony into 10 mL of Tryptic Soy Broth (TSB, Neogen®, United States) followed by incubation for 24 h at 37 °C under anaerobic conditions. Anaerobiosis was achieved by adding a pouch of the AnaeroPack® System (Mitsubishi Gas Chemical America®, United States) into a hermetic chamber. The cultures of C. perfringens were standardized to reach a concentration of 1 × 108 CFU/mL.
An inoculum of C. perfringens was created by diluting the primary culture one thousand-fold (1:1,000) to reach a target concentration of 1 × 105 CFU/mL. Tenfold serial dilutions were completed and plated onto Shahidi-Ferguson Perfringens (SFP) agar (Millipore®, Germany) for the determination of bacterial counts.
An inoculum of C. perfringens was created by diluting the primary culture one thousand-fold (1:1,000) to reach a target concentration of 1 × 105 CFU/mL. Tenfold serial dilutions were completed and plated onto Shahidi-Ferguson Perfringens (SFP) agar (Millipore®, Germany) for the determination of bacterial counts.
4
Synthesis and Characterization of Polymeric Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gelatin powder (type A,
300 bloom), SPH, sodium hydroxide, hydrochloric acid, methanol, ethyl
acetate, hexane, acetone, sodium chloride, potassium chloride, sodium
phosphate dibasic, potassium phosphate monobasic, hydrochloric acid,
glucose, M9 minimal salts (5×), and CV solution (1%) were purchased
from Sigma-Aldrich (Milwaukee, WI). TA was purchased from Alfa Aesar
(Thermo Fisher, Belgium). Anhydrous sodium thiosulfate, 0.1 N iodine
standard solution, and 0.1 N sodium thiosulfate standard solution
were purchased from VWR Chemicals (Radnor, PA). Tryptic soy broth
(TSB) was purchased from Neogen (Lansing, MI), and tryptic soy agar
(TSA) was purchased from bioWorld (Dublin, OH). Tween 20 was purchased
from ChemImpex (Wood Dale, IL). Rifampicin was purchased from Thomas
Scientific LLC (NJ, USA). Tryptone was purchased from Amresco (Solon,
OH). Clorox bleach solution with a free chlorine content of 8.0% was
produced by Clorox Co., Ltd. (Oakland, CA, USA). LDPE sheets were
purchased from the Henta Corporation. DI water was used in the materials
fabrication and tests.
300 bloom), SPH, sodium hydroxide, hydrochloric acid, methanol, ethyl
acetate, hexane, acetone, sodium chloride, potassium chloride, sodium
phosphate dibasic, potassium phosphate monobasic, hydrochloric acid,
glucose, M9 minimal salts (5×), and CV solution (1%) were purchased
from Sigma-Aldrich (Milwaukee, WI). TA was purchased from Alfa Aesar
(Thermo Fisher, Belgium). Anhydrous sodium thiosulfate, 0.1 N iodine
standard solution, and 0.1 N sodium thiosulfate standard solution
were purchased from VWR Chemicals (Radnor, PA). Tryptic soy broth
(TSB) was purchased from Neogen (Lansing, MI), and tryptic soy agar
(TSA) was purchased from bioWorld (Dublin, OH). Tween 20 was purchased
from ChemImpex (Wood Dale, IL). Rifampicin was purchased from Thomas
Scientific LLC (NJ, USA). Tryptone was purchased from Amresco (Solon,
OH). Clorox bleach solution with a free chlorine content of 8.0% was
produced by Clorox Co., Ltd. (Oakland, CA, USA). LDPE sheets were
purchased from the Henta Corporation. DI water was used in the materials
fabrication and tests.
5
Bacterial Strain Preparation and Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The strains used in this study included Salmonella enterica serovar Typhimurium strain ATCC 14028 (S. enterica serovar Typhimurium), Escherichia coli O157:H7 strain ATCC 43895 (E. coli O157:H7), and two deletion mutants of the strain S. enterica serovar Typhimurium, ΔsopD and ΔsseD, obtained as described below by camR and aphA-2 insertions, respectively. The stock cultures were stored at -55°C in a 5:1 solution of Luria-Bertani broth (LB; BBL, Detroit, MI) and glycerol. Working cultures were prepared in tryptic soy broth (TSB, Neogen, Inc., Lansing, MI) or 0.01 M glucose-supplemented LB broth (LBglc) from frozen stock cultures and grown overnight at 37°C shaking at 250 rpm. For mutant strain working cultures, chloramphenicol and kanamycin were added to LBglc to a final concentration of 50 and 100 μg/mL respectively.
6
Salmonella Strain Preparation and Verification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The strains of Salmonella enterica subsp. enterica serovars used in this research were S. Typhimurium E2009005811, S. Tennessee E200700502 and S. Agona. The first two strains were provided by the Minnesota Department of Health; they were isolated from patients linked to the S. Typhimurium E2009005811 and S. Tennessee E200700502 peanut butter outbreaks of 2009 and 2007, respectively [8 ,10 ]. The S. Agona strain was originally isolated from an outbreak related to toasted oats cereal from 1998 [6 ]. The stock cultures of the three serovars were stored in a 1:1 ratio of glycerol and tryptic soy broth (TSB; Neogen, Inc., East Lansing, MI, USA) at −55 °C. The working cultures of each serovar were prepared from frozen stocks and inoculated into TSB, grown overnight at 37 °C and then stored at 4 °C. In order to test the working stocks, they were re-transferred once a week and streaked onto tryptic soy agar (TSA; Neogen, Inc.) containing 0.8 g/L ferric ammonium citrate (Sigma-Aldrich™, St. Louis, MO, USA) and 6.8 g/L sodium thiosulfate. (Acros Organics, Morris Plains, NJ, USA). This medium was formulated to provide a differential but non-selective agar. Periodically, the serovars were also streaked onto bismuth sulfate agar (Neogen, Inc.) and xylose lysine deoxycholate agar (Neogen, Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!