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Falcon 50 ml conical centrifuge tubes

Manufactured by Corning
Sourced in United States

The Falcon™ 50 ml conical centrifuge tubes are a laboratory equipment product designed for use in centrifugation applications. They have a capacity of 50 milliliters and a conical shape.

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2 protocols using falcon 50 ml conical centrifuge tubes

1

Clip Cage Design for Insect-Leaf Interactions

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The clip cages used are a modified version of the MacGillivray and Anderson clip cage [42 (link)], and are also similar in design to clip cages used by other authors [43 ]. Clip cages are constructed out of two Corning™ Falcon™ 50 ml conical centrifuge tubes (Corning Incorporated, NY, NY, USA) with the bottom of the tubes cut off and separated by fine mesh (Figure S1 A). This mesh allows air flow to the leaf and the caged insects. The leaf clips interface with the leaf surface on the insect cage side with a modified Falcon™ tube cap with a 24 mm hole cut into it, and that is pressed against a 35 mm plastic disc on the opposite side of the leaf. 4.76 mm thick polyurethane foam (76 Flex-Foam™, Pellon, Clearwater, FL, USA) is affixed to both sides these plastic parts to provide a good seal to prevent insect escape without damaging the leaf (Figure S1 B). An aluminum hair sectioning clip (Goody Products, Atlanta, GA, USA) holds the disc and cap pieces together and applies pressure to seal the foam against the leaf (Figure S1 C). Further details on clip cage design and construction is provided in Supplemental Methods and in Figure S1.
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2

Droplet Digital PCR for HPV Detection

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Droplet digital PCR reagents (2x ddPCR™ Supermix for Probes (No dUTP) Catalog # 186–3024; Pipet tips Catalog # 186–4121; Cartridge Catalog # 186–4109 and Sealing foil Catalog #181–4040) were purchased from Bio-Rad (Hercules, CA). Eppendorf™ 96-Well twin.tec™ PCR Plates (Catalog #E951020362), Falcon® 15ml Conical Centrifuge Tubes (Catalog # 352096), Falcon™ 50mL Conical Centrifuge Tubes (Corning #352098), Qubit™ dsDNA HS Assay Kit (Catalog # Q32851), and Qubit™ Assay Tubes (Catalog # Q32856) were purchased from Fisher scientific (Fair Hampton, NH). Bovine Serum Albumin was from Sigma (Catalog # A7906; Sigma–Aldrich, St. Louis, MO). Circulating DNA was quantified with Qubit (Invitrogen, Carlsbad, CA, USA). Target gene fragments for HPV subtype 16, 18, 31, 33, and 35 were synthesized as gBlocks from IDT for use as positive controls (Supplementary Table S1). These gBlocks were cloned into a pCR2.1-TOPO TA vector by Topo TA cloning kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. The gene fragment constructs were verified by Sanger sequencing (Genewiz, New Jersey, USA). FAM-, TET- or HEX-conjugated Locked Nucleic Acids (LNA)-modified DNA oligonucleotide probes and FAM-ZEN dual quencher probes were synthesized by IDT (Integrated DNA Technology, San Jose, CA, see Supplementary Table S1).
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