Alexa fluor 700 mouse anti cleaved parp asp214

BTZ, Cell Counting Kit-8, and N-acetylcysteine (NAC) were purchased from Sigma Aldrich (St. Louis, MO, USA). z-VAD-FMK was purchased from Calbiochem (San Diego, CA, USA). Antibodies against Skp2, p21, p27, MTH1, CDK4, CDK6, caspase-9, Bcl-2, cleaved caspase-3, caspase-3, Bax, p53, Cytochrome c, PARP, Cleaved caspase-8, PH2AX were purchased from Cell Signaling Technology (Danvers, MA, USA). FITC Annexin V apoptosis detection kit I, Apo-Direct kit, Fixation/Permeabilization solution kit, BD MitoScreen (JC1), BV421 mouse anti-γH2AX (pS139), PE rabbit anti-active caspase-3, and Alexa Fluor 700 mouse anti-cleaved PARP (Asp214) antibodies were purchased from BD Biosciences (San Jose, USA). CellROXGreen was purchased from Invitrogen (Massachusetts, USA).

Cell Counting Kit‐8 (CCK‐8) was purchased from Sigma Aldrich. z‐VAD‐fmk and BTZ were purchased from Tocris. Antibodies against SKP2, P21, P27, CDK‐4, CDK‐6, caspase‐9, cleaved caspase‐3, caspase‐3, Bid, Poly(ADP‐ribose) polymerases (PARP), cleaved caspase 8, cleaved caspase 9, XIAP, CIAP1 and 2, P‐AKT, AKT, ubiquitin, cyclin D1 and B1 were purchased from Cell Signaling Technologies. Antibodies against HSP60, p‐H2AX, Bax, cytochrome c, and Bcl2 were purchased from Santa Cruz Biotechnology Inc. FBS, penicillin–streptomycin, live and dead assay kit, and RPMI 1640 medium were purchased from Life Technologies Inc. Fluorescein isothiocyanate (FITC) Annexin V apoptosis detection kit I, Apo‐Direct kit, fixation/permeabilization solution kit, BD MitoScreen (JC‐1), BV421mouse anti‐p‐H2AX (pS139), PE rabbit antiactive caspase‐3, and Alexa Fluor 700 mouse anticleaved PARP (Asp214) antibodies were purchased from BD Biosciences.

Sanguinarine chloride, Cell Counting Kit-8 (CCK-8), and N-acetylcysteine (NAC) were purchased from Sigma Chemical Co. (St. Louis, USA). Z-VAD-FMK was purchased from Calbiochem (San Diego, USA). Antibodies against caspase-9, Bclxl, Bcl2, phospho-STAT3, STAT3, SHP-1, cleaved caspase-3, and caspase-3 were purchased from Cell Signaling Technologies (Beverly, USA). VELCADE® (Bortezomib), PARP, and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, USA). XIAP antibody was purchased from Abcam (Cambridge, UK). FITC Annexin V apoptosis detection kit I, Apo-Direct kit, Fixation/Permeabilization solution kit, BD MitoScreen (JC-1), BV421 mouse anti-γH2AX (pS139), PE rabbit anti-active caspase-3, and Alexa Fluor 700 mouse anti-cleaved PARP (Asp214) antibodies were purchased from BD Biosciences (San Jose, USA). CellROXGreen and ThiolTracker Violet were purchased from Invitrogen (Massachusetts, USA). RPMI 1640, fetal bovine serum (FBS), Penicillin Streptomycin (PenStrep) were purchased from Life technologies (California, USA).