Abi prism 7300 system

Manufactured by PerkinElmer

Sourced in United Kingdom

The ABI PRISM 7300 system is a real-time PCR instrument designed for gene expression analysis and quantification. It enables precise measurement of DNA or RNA targets and provides accurate quantification of gene expression levels.

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Lab products found in correlation

Cdna synthesis kit, by Takara Bio (2 mentions) Rnase free dnase, by Promega (1 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Tripure isolation reagent, by Roche (1 mentions) Sybr green reagent, by Thermo Fisher Scientific (1 mentions) Abi prism sds 2, by PerkinElmer (1 mentions) Trizol kit, by Thermo Fisher Scientific (1 mentions) Sybr green pcr core reagent, by Takara Bio (1 mentions) Dnase 1, by Promega (1 mentions)

3 protocols using abi prism 7300 system

Quantitative Transcriptome Analysis Workflow

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Total RNA of cells was extracted by TriPure Isolation Reagent (Roche, Basel, Switzerland), followed by RNase-free DNase (Promega, Madison, WI, USA) treatment. Complementary DNA (cDNA) synthesis kit (Takara, Dalian, Liaoning, China) was utilized to synthesize cDNA according to the manufacturer’s instructions. Polymerase chain reaction (PCR) amplifications of the respective genes were carried out with Power SYBR Green PCR Master mix (Applied Biosystems, Warrington, UK) using the ABI PRISM 7300 system (Perkin-Elmer, Torrance, CA, USA). Each reaction was repeated at least three times independently. Sequences of PCR primers used in this study are shown in Table 1.

Zhao X.Y., Li L., Wang X.B., Fu R.J., Lv Y.P., Jin W., Meng C., Chen G.Q., Huang L, & Zhao K.W. (2016). Inhibition of Snail Family Transcriptional Repressor 2 (SNAI2) Enhances Multidrug Resistance of Hepatocellular Carcinoma Cells. PLoS ONE, 11(10), e0164752.

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Genotyping and Quantitative PCR for Mice

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For mice genotyping, we isolated genomic DNA from tail clips using DiretPCR lysis reagent (Viagen) and determined the genotypes of SENP2^fxN/fxN, SENP2^fx/fx -Thy1, and SENP2^fx/fx -MHC using PCR amplification of specific alleles with indicated primers (Table S2). Quantitative PCR was then performed using reaction mixtures of 20 ng total RNA, 100 nM primers (Table S3), and SYBR Green reagent (Applied Biosystems) with the ABI PRISM 7300 system (Perkin-Elmer). PCR was done in triplicate, and SDs representing experimental errors were calculated. All data were analyzed using ABI PRISM SDS 2.0 software (Perkin-Elmer).

Qi Y., Wang J., Bomben V.C., Li D.P., Chen S.R., Sun H., Xi Y., Reed J.G., Cheng J., Pan H.L., Noebels J.L, & Yeh E.T. (2014). Hyper-SUMOylation of the Kv7 Potassium Channel Diminishes the M-Current Leading to Seizures and Sudden Death. Neuron, 83(5), 1159-1171.

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Quantitative RT-PCR for Gene Expression Analysis

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Total RNA was isolated by Trizol kit (Invitrogen). RNA was treated with DNase I (Promega). Complementary DNA was synthesized using the cDNA synthesis kit (Takara) according to the manufacturer's instructions. Fluorescence real-time RT-PCR was performed with the double-stranded DNA dye SYBR Green PCR Core Reagents (Takara) using the ABI PRISM 7300 system (Perkin-Elmer). PCR was done in triplicate and standard deviations representing experimental errors were calculated. Pairs of PCR primers used to amplification of the target genes were as Table 1.

Chen B., Luo J., Zhou Y., Xin X., Cai R, & Ling C. (2018). PIASy antagonizes Ras-driven NSCLC survival by promoting GATA2 SUMOylation. Journal of Cancer, 9(9), 1689-1697.

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