Las v4.4 system software

Manufactured by Leica

Sourced in Germany

LAS v4.4 is a system software developed by Leica for laboratory equipment. It serves as the core operating software, providing fundamental functionalities to enable the equipment's intended functions.

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Lab products found in correlation

Orca flash4.0 camera, by Leica (3 mentions) Dm2000 microscope, by Leica (3 mentions) Las x software v2, by Leica (3 mentions) Dm5500b microscope, by Hamamatsu Photonics (1 mentions) Lasaf v2, by Leica (1 mentions) Tcs sp5 2, by Leica (1 mentions) Dfc450 camera, by Leica (1 mentions) Lasx software package, by Leica (1 mentions)

Close spelling variants of this product

LAS software V4.8 Leica LAS 4.3 LAS software Leica software system

4 protocols using las v4.4 system software

Meiotic Metaphase I Nuclei Imaging

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Meiotic metaphase I nuclei stained with acetocarmine or Feulgen were imaged using a Leica DM2000 microscope equipped with a Leica DFC450 camera and controlled by LAS v4.4 system software (Leica Biosystems, Wetzlar, Germany). Images were processed using Adobe Photoshop CS5 (Adobe Systems Incorporated, USa) extended version 12.0 × 64.
Meiotic metaphase I nuclei labelled by GISH were imaged using a Leica DM5500B microscope equipped with a Hamamatsu ORCA-FLASH4.0 camera and controlled by Leica LAS X software v2.0. Images were processed using Fiji (an implementation of ImageJ, a public domain program by W. Rasband available from http://rsb.info.nih.gov/ij/).
Polyacrylamide-embedded meiocytes were optically sectioned using a Leica TCS SP5II confocal laser scanning microscope (CLSM) controlled by Leica LAS-AF v.2.7 software. Z-stacks were deconvolved using Huygens Essential (Scientific Volume Imaging BV). Projections and analysis of 3D pictures were performed using Fiji.

Martín A.C., Rey M.D., Shaw P, & Moore G. (2017). Dual effect of the wheat Ph1 locus on chromosome synapsis and crossover. Chromosoma, 126(6), 669-680.

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Cytological Analysis of Meiotic Metaphase I

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A total of five plants per line were examined in a randomised block design. Generally, 2–3 tillers in each plant were analysed to identify meiocytes at meiotic metaphase I. Anthers from Tazip4-B2 Cadenza mutant lines, wild-type Cadenza, Tazip4-B2 mutant line-Ae variabilis hybrids, and Cadenza-Ae. variabilis hybrids were harvested as described in the “Plant material” section. Cytological analysis of meiocytes was performed using Feulgen reagent as previously described (Sharma and Sharma 2014 ). Images were collected using a LEICA DM2000 microscope (LeicaMicrosystems, http://www.leica-microsystems.com/), equipped with a Leica DFC450 camera and controlled by LAS v4.4 system software (Leica Biosystems, Wetzlar, Germany). Images were processed using Adobe Photoshop CS5 (Adobe Systems Incorporated, US) extended version 12.0 × 64.

Rey M.D., Martín A.C., Higgins J., Swarbreck D., Uauy C., Shaw P, & Moore G. (2017). Exploiting the ZIP4 homologue within the wheat Ph1 locus has identified two lines exhibiting homoeologous crossover in wheat-wild relative hybrids. Molecular Breeding, 37(8), 95.

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Feulgen-stained Pollen Mother Cell Imaging

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Pollen Mother Cells stained by the Feulgen technique were imaged using a LEICA DM2000 microscope (Leica Microsystems, http://www.leica-microsystems.com/), equipped with a Leica DFC450 camera and controlled by LAS v4.4 system software (Leica Biosystems, Wetzlar, Germany).
All cells labelled by GISH were imaged using a Leica DM5500B microscope equipped with a Hamamatsu ORCA-FLASH4.0 camera and controlled by Leica LAS X software v2.0. Images were processed using Adobe Photoshop CS5 (Adobe Systems Incorporated, US) extended version 12.0 × 64.

Martín A.C., Alabdullah A.K, & Moore G. (2021). A separation-of-function ZIP4 wheat mutant allows crossover between related chromosomes and is meiotically stable. Scientific Reports, 11, 21811.

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Feulgen-stained Pollen Microscopy

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Pollen grains and Pollen Mother Cells stained by the Feulgen technique were imaged using a LEICA DM2000 microscope (Leica Microsystems, http://www.leica-microsystems.com/, accessed on 31 March 2021), equipped with a Leica DFC450 camera and controlled by LAS v4.4 system software (Leica Biosystems, Wetzlar, Germany). Tetrads labelled by FISH were imaged using a Leica DM5500B microscope equipped with a Hamamatsu ORCA-FLASH4.0 camera and controlled by Leica LAS X software v2.0. Z-stacks were processed using the 561-deconvolution module of the Leica LAS X Software package. Images were processed using Adobe Photoshop CS5 (Adobe Systems Incorporated, San Jose, CA, USA) extended version 12.0 × 64.

Alabdullah A.K., Moore G, & Martín A.C. (2021). A Duplicated Copy of the Meiotic Gene ZIP4 Preserves up to 50% Pollen Viability and Grain Number in Polyploid Wheat. Biology, 10(4), 290.

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