To investigate the role of ET‐1 plays in pathological hypertension, additional groups of eight‐ and thirteen‐week‐old SHRs (n = 4 in each group) were treated with an ET‐1 receptor antagonist. Briefly, the mesentery was incubated with 10 μM bosentan, an ET‐1 receptor antagonist (Med Chem Express, Monmouth Junction, NJ, USA) for 30 min. As mentioned above, lymphatic microcirculatory data were collected before and after 10 μM bosentan incubation were collected and analyzed. The lymphatic vasomotion profile, contractility and reflux were compared.
Bosentan
Manufactured by MedChemExpress
Sourced in United States
Bosentan is a laboratory equipment product offered by MedChemExpress. It is an endothelin receptor antagonist used in research settings. The core function of Bosentan is to inhibit the binding of endothelin to its receptors.
Automatically generated - may contain errors
Lab products found in correlation
B6.129s7 ldlrtm1her j, by Jackson ImmunoResearch (1 mentions)
C57bl 6j, by Jackson ImmunoResearch (1 mentions)
Cell count reagent sf, by Nacalai Tesque (1 mentions)
Nivo microplate reader, by PerkinElmer (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Calponin 1, by Abcam (1 mentions)
Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (1 mentions)
High glucose dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Hif 1α, by Proteintech (1 mentions)
Pdgf bb, by MedChemExpress (1 mentions)
α smooth muscle actin α sma, by Abcam (1 mentions)
Wogonin, by MedChemExpress (1 mentions)
Escherichia coli lps, by Merck Group (1 mentions)
Sp600125, by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
Dispase 2, by Roche (1 mentions)
Pd98059, by Merck Group (1 mentions)
U73122, by Merck Group (1 mentions)
Collagenase a, by Roche (1 mentions)
P gingivalis lps, by InvivoGen (1 mentions)
5 protocols using bosentan
1
Role of ET-1 in Hypertension Pathogenesis
2
LDLr-Knockout Mice on Western Diet Treated with Bosentan
3
Cell Viability Assay under Stress Conditions
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Cells were treated with DMEM/high glucose (4.5 g/L) media containing 0.1% fetal bovine serum, DMEM/glucose-free media (09891-25; Nacalai Tesque) containing 0.1% fetal bovine serum, or DMEM/high glucose media containing 0.1% fetal bovine serum with tunicamycin (TM) (0.2 µg/mL, 35638-74; Nacalai Tesque). To assess cell viability, cells were cultured in the aforementioned medium with or without KUS121 (100 µM) and with bosentan (0; 100; 500; 2500 nM; MedChemExpress LLC, Monmouth Junction, NJ, USA), and/or endothelin-1 (0, 1, 10, 100 nM; Peptide, Osaka, Japan) and incubated at 37 °C for 40 h (under TM-stress conditions) or 48 h (under glucose-free conditions). In experiments involving 661W cells, KUS was added simultaneously with the stressor.
For cell viability assays, cells were washed using Dulbecco’s phosphate-buffered saline and incubated with Cell Count Reagent SF (07553-15; Nacalai Tesque) for 20 min. Using a Nivo Microplate reader (PerkinElmer, Waltham, MA, USA), the relative amount of live cells was subsequently determined by measuring the absorbance at 450 nm of the formazan produced by WST-8 reduction via intracellular dehydrogenase.
For cell viability assays, cells were washed using Dulbecco’s phosphate-buffered saline and incubated with Cell Count Reagent SF (07553-15; Nacalai Tesque) for 20 min. Using a Nivo Microplate reader (PerkinElmer, Waltham, MA, USA), the relative amount of live cells was subsequently determined by measuring the absorbance at 450 nm of the formazan produced by WST-8 reduction via intracellular dehydrogenase.
4
Investigating Endothelial Cell Responses
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Wogonin, bosentan, and PDGF-BB were purchased from MedChemExpress (Israel Shekel). High-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Thermo Fisher Scientific Inc., Waltham, MA, USA). Phalloidin and Hochest 33342 were purchased from Abcam (Cambridge, UK). Primary antibodies specific for vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), and HIF-1α were procured from ProteinTech (Chicago, IL, USA). Primary antibodies against NOX4, α-smooth muscle actin (α-SMA), and calponin 1 were purchased from Abcam (Cambridge, UK). The Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Laboratories (Kumamoto, Japan). Other reagents and chemicals were all analytical grade.
5
Keratinocyte Growth and Characterization
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Keratinocyte Basal Medium-2 (KBM-2) was purchased from Lonza (Walkersville, MD, USA). Collagenase A and Dispase II were obtained from Roche (Mannheim, Germany). P. gingivalis, P. gingivalis LPS, P. gingivalis LPS-ulp, HKLM and Pam3 were purchased from Invivogen (San Diego, CA, USA). BQ123 and BQ788 were obtained from American Peptides Company (Sunnyvale, CA, USA). Bosentan was a product of MedChem Express (Monmouth Junction, NJ, USA). U73122, U73433, PD98059, SP600125, SB203580, 2-aminoethoxydiphenyl borate (2-APB), 1,2-Bis(2-aminophenoxy)ethane-N, N, N’, N’-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM) and Escherichia coli LPS were products of Sigma-Aldrich (St. Louis, MO, USA).
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