Bay61 3606

Seven week-old male mice (n = 4 per group) (C57BL/6 background) were purchased from Samtako Bio (Osan, Korea). On the first week, mice were housed in a facility providing automatic 12 h light/12 h dark cycle, humidity (60 ± 15%), and temperature (23 ± 2 °C) for assimilation. Mice were fed ad libitum food and water. The groups were divided as shown below, and all chemicals (LPS (L2630, Sigma Aldrich, St.Louis, MO, USA), BAY61-3606 (11423, Cayman, Ann Arbor, MI, USA)) were treated by intraperitoneal injection. Figure 1B describes the overall schematic of the experimental schedules.

Anti-p-p65 antibody, Gö6976, heparin, PMA, Röttlerin, and PKC-δ siRNA were purchased from Sigma-Aldrich (St.Louis, MO). Bay 11-7082, Bay 61-3606, piceatannol, and caffeic acid phenethyl ester (CAPE) were purchased from Cayman Chemicals (Ann Arbor, MI). Lipofectamine 3000 reagent was acquired from Invitrogen Life Technologies (Gaithersburg, MD). Antibody specific for Thy-1, and p-PKC-δ was purchased from Cell Signaling Technology (Danvers, MA). Anti-Syk, p65, PKC-δ, and G3PDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody specific for p-Syk was purchased from EMD Millipore (Temecula, CA). Dual-Luciferase assay kit was purchased from Promega (Madison, WI). Penicillin and streptomycin were purchased from HyClone (South Logan, Utah). Endothelium cell growth factor (ECGS) was purchased from Biomedical Technologies (Stoughton, MA). Fetal calf serum (FCS), Medium 199 (M199), and GlutaMAX Supplement were obtained from GIBCO (Grand Island, NY). Endothelial Cell Growth Medium MV was purchased from PromoCell (Heidelberg, Germany).

BAY61-3606 was obtained from Cayman Chemical (CAY11423), dissolved in DMSO, and aliquoted into single-use aliquots prior to use. P505-15 (also known as PRT062607) was from MedChem Express (HY-15323) in DMSO-dissolved format and was aliquoted into single-use aliquots prior to use.