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Donkey anti rabbit fitc cy3 conjugated secondary mab

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Donkey anti-rabbit FITC/CY3 conjugated secondary mAb is a laboratory reagent used for the detection of rabbit primary antibodies in immunological assays. It contains donkey-derived secondary antibodies conjugated with fluorescent labels FITC and CY3.

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Lab products found in correlation

Ab7417, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Ab86299, by Abcam (1 mentions) D9m8i, by Cell Signaling Technology (1 mentions) Ab75769, by Abcam (1 mentions) Facscalibur cytometer, by BD (1 mentions)

Spelling variants of this product

Anti-rabbit Cy3 (11 citations) Donkey anti-rabbit FITC (4 citations)

2 protocols using donkey anti rabbit fitc cy3 conjugated secondary mab

1

Immunofluorescence Analysis of hJBMMSCs

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Cultured hJBMMSCs were stained by immunofluorescence after treatment. Primary phospho-P65 mAb (ab86299, Abcam, Cambridge, United Kingdom), CD29 mAb (303005, Biolegend, San Diego, CA) or CD13 mAb (ab7417, Abcam) were used and followed by donkey anti-rabbit FITC/CY3 conjugated secondary mAb (Life Technologies, Grand Island, NY). Cells were treated with DAPI (Vector Labs), and evaluated blindly by counting labeled cells in ten HPFs (high-power fields).
Chen X., Liu Y., Ding W., Shi J., Li S., Liu Y., Wu M, & Wang H. (2018). Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-κB pathway. Cell Death & Disease, 9(2), 207.
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2

Characterization of hJBMMSCs

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Cultured hJBMMSCs were isolated and stained with primary CD29 mAb (303005, Biolegend), CD13 mAb (ab7417, Abcam), CD45 mAb (D9M8I, Cell Signaling Technology, Beverly, MA), and CD146 mAb (ab75769, Abcam), which was followed by donkey anti-rabbit FITC/CY3 conjugated secondary mAb (Life Technologies). hJBMMSCs were then analyzed on a FACSCalibur cytometer (BD Biosciences) and identified as CD29+CD13+CD45−CD146−.
Chen X., Liu Y., Ding W., Shi J., Li S., Liu Y., Wu M, & Wang H. (2018). Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-κB pathway. Cell Death & Disease, 9(2), 207.
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