Mouse monoclonal anti calnexin

HA was a kind gift from Prof. Ivan Donati, Department of Life Sciences, University of Trieste. The following antibodies were used: mouse monoclonal anti-vimentin, mouse monoclonal anti-LAMP1, mouse monoclonal anti-HAS2 (#sc-365263) and mouse monoclonal anti-actin purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-EEA1, mouse monoclonal anti-Rab11A, mouse monoclonal anti-calnexin, mouse monoclonal anti-TGN38, streptavidin Cy3-conjugated and anti-mouse IgG fluorescein isothiocyanate (FITC)-conjugated from Abcam (Abcam Inc, Toronto, ON, Canada); rabbit polyclonal anti-C1q from Dako (Dako, Glostrup, Denmark); rabbit polyclonal anti-HAS1 (#PA5-50674) from Invitrogen^TM (Thermo Fisher Scientific, Waltham, MA, USA); rabbit polyclonal anti-HAS3 (#15609-1-AP) from Proteintech (Proteintech, Rosemount, IL, USA); anti-mouse LI-COR IRDye 680RD and anti-rabbit LI-COR IRDye 800CW from LI-COR Biosciences (LI-COR Biosciences, Lincoln, NE, USA). All chemicals were purchased from Sigma (Sigma-Aldrich, Saint Louis, MO, USA).

Primary antibodies used in this study include the following: rabbit polyclonal anti-tricellulin (c-term), mouse monoclonal anti-occludin (Zymed Laboratories, San Francisco, CA); rabbit polyclonal anti-actin (Sigma-Aldrich, St Louis, MO); goat polyclonal anti-LDH (as a marker enzyme of cytosolic fraction), mouse monoclonal anti-calnexin (as a marker of the membrane fraction) (Abcam, Cambridge, MA); mouse monoclonal anti-PAPR-1 (as a marker of the nuclear fraction) (Calbiochem). Twelve-nm colloidal gold-conjugated anti-rabbit IgG was purchased from Jackson Immuno Research Laboratories (Western Grove, PA). The secondary antibodies used were horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse immunoglobulin (Ig)G (Dako ChemMate, Glostrup, Denmark), Alexia Flour 488 (green)-labeled anti-rabbit or anti-mouse IgG (Invitrogen, Carlsbad, CA), and Alexa Flour 594 (red)-labeled anti-rabbit or anti-mouse IgG (Invitrogen). DAPI was obtained from Sigma-Aldrich and Hoechst 33342 solution was obtained from Dojindo (Kumamoto, Japan).

Antibodies for immunoblot (Western) were as follows: mouse monoclonal anti-cytochrome C (1:500 dilution; BD Biosciences; RRID: AB_396417); mouse monoclonal anti-calnexin (1:1000 dilution; Abcam; RRID: AB_11178981); rabbit monoclonal anti-syntenin (1:2500 dilution; Abcam; RRID: AB_11160262); rabbit monoclonal anti-GM130 (1:2500 dilution; Abcam; RRID: AB_880266); rabbit monoclonal anti-CD81 (1:1000 dilution; Cell Signaling; RRID: AB_2714207); mouse monoclonal anti-flotillin-1 (1:500 dilution; BD Transduction Laboratories; RRID: AB_398139); rabbit polyclonal anti-cyclophilin A/peptidyl-prolyl cis-trans isomerase A (PPIA) (1:5000 dilution; Proteintech; RRID: AB_2237516); rabbit polyclonal anti-HSP90 (1:1000 dilution; Enzo Life Sciences; RRID: AB_2039287); rabbit polyclonal anti-TDP-43 (1:2500 dilution; Proteintech; RRID: AB_2200505); rabbit polyclonal anti-TDP-43 (1:2500 dilution; Proteintech; RRID: AB_615042); mouse monoclonal anti-human phospho Ser409/410 TDP-43 antibody (pTDP-43) (1:2000 dilution; Cosmo Bio Co., LTD; RRID: AB_1961900); goat polyclonal anti-apoliprotein B (1:500 dilution; Abcam; RRID: AB_305987); goat polyclonal anti-apoliprotein AI (1:500 dilution; Abcam; RRID: AB_2289632); goat anti-mouse or anti-rabbit peroxidase-conjugated secondary antibodies (respectively 1:20,000 and 1:10,000 dilution, GE Healthcare).