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Fei tf20

Manufactured by Thermo Fisher Scientific
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The FEI TF20 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a field emission electron source, advanced optics, and high-performance detectors to enable detailed characterization of nanostructures and thin films.

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Lab products found in correlation

Fei morgagni, by Thermo Fisher Scientific (2 mentions) Us4000 4kx4k ccd camera, by Ametek (1 mentions) Us4000 4k 4k ccd camera, by Ametek (1 mentions)

Spelling variants of this product

FEI Tecnai TF20 FEG transmission electron microscope (2 citations) FEI Tecnai TF20 FEG electron microscope (2 citations) TF20 FEI Tecnai-G2 (2 citations) FEI Tecnai TF20 (2 citations)

2 protocols using fei tf20

1

Cryo-EM Sample Imaging and Processing

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NS grids were screened on an FEI Morgagni (Thermo Fisher Scientific) microscope operating at 100kV with AMT 1k×1k CCD camera to verify sample and grid quality. Data collection from NS grids were done on FEI TF20 (Thermo Fisher Scientific) operate at 200kV with US4000 4kx4k CCD camera (Gatan) and controlled by SerialEM.69 (link) The dataset was collected at nominal mag of 50Kx with A/pix of 2.18 with defocus range of 1.4–1.8 and a total dose of ~30.0e/A2. Image processing was performed using the CryoSPARC software package.70 (link) The dataset was imported, CTF estimated, and particles were picked. The particles were extracted with box size of 256 × 256 pixels and 2D classification was performed to generated clean homogeneous classes.
Pilewski K.A., Wall S., Richardson S.I., Manamela N.P., Clark K., Hermanus T., Binshtein E., Venkat R., Sautto G.A., Kramer K.J., Shiakolas A.R., Setliff I., Salas J., Mapengo R.E., Suryadevara N., Brannon J.R., Beebout C.J., Parks R., Raju N., Frumento N., Walker L.M., Fechter E.F., Qin J.S., Murji A.A., Janowska K., Thakur B., Lindenberger J., May A.J., Huang X., Sammour S., Acharya P., Carnahan R.H., Ross T.M., Haynes B.F., Hadjifrangiskou M., Crowe JE J.r., Bailey J.R., Kalams S., Morris L, & Georgiev I.S. (2023). Functional HIV-1/HCV cross-reactive antibodies isolated from a chronically co-infected donor. Cell reports, 42(2), 112044.
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2

Cryo-EM Structural Analysis of Macromolecular Complexes

Cited 1 time
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NS grids were screened on a FEI Morgagni (ThermoFisher, Waltham, MA, USA) microscope operating at 100 kV with AMT 1k × 1k CCD camera to verify sample and grid quality. Data collection from NS grids were recorded on FEI TF20 (Thermo Fisher, Waltham, MA, USA) operate at 200 kV with US4000 4k × 4k CCD camera (Gatan, Pleasanton, CA, USA) and controlled by SerialEM [54 ]. The data set was collected at mag of 50 K with Å/pix of 2.18 with defocus range of 1.4 to 1.8 and a dose rate of ~30.0 e/A2. 120 micrographs were collected and process in Scipion [55 (link)]. The data set was binned to 4.36 Å/pix and 3,357 particles was autopicked and extract at a box size of 128 pixel. Multiple rounds of 2D classification [55 (link)–57 (link)] were performed to clean the dataset and separate the different species into distinct classes.
Schoeder C.T., Gilchuk P., Sangha A.K., Ledwitch K.V., Malherbe D.C., Zhang X., Binshtein E., Williamson L.E., Martina C.E., Dong J., Armstrong E., Sutton R., Nargi R., Rodriguez J., Kuzmina N., Fiala B., King N.P., Bukreyev A., Crowe JE J.r, & Meiler J. (2022). Epitope-focused immunogen design based on the ebolavirus glycoprotein HR2-MPER region. PLoS Pathogens, 18(5), e1010518.
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