Nzb binj

Manufactured by Jackson ImmunoResearch

Sourced in United States

The NZB/BINJ is a laboratory reagent produced by Jackson ImmunoResearch. It is a secondary antibody that can be used to detect the presence of specific proteins or molecules in a sample. The core function of NZB/BINJ is to bind to and label the target protein or molecule, enabling its detection and quantification in various analytical techniques.

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Lab products found in correlation

6 protocols using nzb binj

Mouse Diversity Panel Protocol

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Thirty-six male inbred mouse strains (129S1/SvImJ, 129X1/SvJ, A/J, AKR/J, BALB/cByJ, BTBR T⁺Itpr3^tf/J, BUB/BnJ, C3H/HeJ, C57BLKS/J, C57BL/6J, C57BR/cdJ, C58/J, CBA/J, CZECHII/EiJ, DBA/2J, FVB/NJ, I/LnJ, KK/HiJ, LG/J, LP/J, MA/MyJ, NOD/LtJ, NON/LtJ, NZB/BINJ, NZO/HiLtJ, NZW/LacJ, PERA/EiJ, PL/J, PWD/PhJ, PWK/PhJ, RIIIS/J, SEA/GnJ, SJL/J, SM/J, SWR/J, and WSB/EiJ), aged 10–12 weeks, were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). This panel of isogenic mice was chosen based on priority strains from the Mouse Diversity Panel.26 (link) Four mice were used per strain. Male mice were housed four per cage in polycarbonate cages on a 12-hour light/dark cycle (lights on at 7 am), with access to food and water ad libitum. All procedures were approved by the Institutional Animal Care and Use Committee and followed the guidelines set forth by the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals.

Frick A., Fedoriw Y., Richards K., Damania B., Parks B., Suzuki O., Benton C.S., Chan E., Thomas R.S, & Wiltshire T. (2015). Immune cell-based screening assay for response to anticancer agents: applications in pharmacogenomics. Pharmacogenomics and Personalized Medicine, 8, 81-98.

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Murine Lupus Model Characterization

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NZB/BINJ and NZW/LacJ were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). These lines were maintained and interbred to generate F1 generation (BWF1) in the Minimal Disease Area of the Laboratory Animal Unit, The University of Hong Kong. Disease development in BWF1 was assessed by monitoring serum level of anti-dsDNA IgG and development of proteinuria, as previously described [27 (link)]. Mice between 8 and15 weeks old without an increase in anti-dsDNA IgG (level comparable to the non-lupus parental strain NZW) and negative for proteinuria were defined as pre-symptomatic mice, while mice above 20 weeks old with elevation of anti-dsDNA IgG (level higher than two standard deviations of mean serum level in NZW) and proteinuria development (3mg/mL or above) for at least two consecutive weeks were defined as symptomatic mice. Age- and sex-matched non-lupus parental strain NZW was used as controls in specified experiments (young NZW between 11 and 15 weeks old, old NZW between 30 and 40 weeks old). All animal experiments were approved by the Committee on the Use of Live Animal in Teaching and Research (CULATR, approval number 3412-14 and 3647-15), the University of Hong Kong.

Yim L.Y., Lau C.S, & Chan V.S. (2019). Heightened TLR7/9-Induced IL-10 and CXCL13 Production with Dysregulated NF-ҝB Activation in CD11chiCD11b+ Dendritic Cells in NZB/W F1 Mice. International Journal of Molecular Sciences, 20(18), 4639.

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Genetic Background in Drug Abuse Sensitivity

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Male 129S1/SvlmJ, 129S4/SvJaeJ, 129S8/SvEvNimrJ, A/J, AKR/J, BALB/cJ, BTBRT < +>ltpr3 < tf>/J, C3H/HeJ, C57BL/6J, CBA/J, DBA/1J, DBA/2J, FVB/NJ, LP/J, MA/MyJ, NZB/BINJ, SJL/J, SM/J, & SWR/J mice were obtained from Jackson Laboratory (Bar Harbor, ME). 129S2/SvPasCrl were obtained from Charles River (Wilmington, MA). Individual strain characteristics can be found on https://mice.jax.org/ and https://www.criver.com/ (129S2). These mice were part of a larger project examining the influence of genetic background on sensitivity to drugs of abuse. All mice were 10–15 weeks of age for behavioral testing and tissue collection (n = 9–13 per strain). All mice were group-housed [with the exception of SJL/J, which were single-housed due to excessive social aggression characteristic of this strain; (43 (link))] with a 12-h light/dark cycle and unlimited access to food and water. All behavioral testing occurred between 8:00 A.M. and 5:00 P.M. All procedures were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals and approved by the Penn State University IACUC Committee.

Mooney-Leber S.M., Zeid D., Garcia-Trevizo P., Seemiller L.R., Bogue M.A., Grubb S.C., Peltz G, & Gould T.J. (2021). Genetic Differences in Dorsal Hippocampus Acetylcholinesterase Activity Predict Contextual Fear Learning Across Inbred Mouse Strains. Frontiers in Psychiatry, 12, 737897.

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ENU-Induced Mutagenesis in C57BL/6J Mice

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Eight- to ten-week old pure C57BL/6J background males purchased from The Jackson Laboratory were mutagenized with N-ethyl-N-nitrosourea (ENU) as described previously (20 (link)). Mutagenized G0 males were bred to C57BL/6J females, and the resulting G1 males were crossed to C57BL/6J females to produce G2 mice. G2 females were backcrossed to their G1 sires to yield G3 mice, which were screened for phenotypes. Whole-exome sequencing and mapping were performed as described (20 (link)). C57BL/6.SJL (CD45.1; #002014) (57 (link)), Rag2^−/− (#008449) (58 (link)), Tcra^−/− (#002116) (59 (link)), muMT (#002288) (60 (link)), Bcl6^fl/fl (#023727) (61 (link)), Il12a^−/− (#002692) (62 (link)), NZB/BINJ (#000684) (63 (link)), NZW/LacJ, (#001058) (64 (link)) and CD4-Cre transgenic mice (#017336) (65 (link)) were purchased from The Jackson Laboratory. 4-get reporter mice were purchased from genOway. Prkd2^−/−/4-get, Prkd2^−/−Tcra^−/−, Prkd2^−/−Bcl6^fl/flCD4-Cre⁺, and NZB/NZW F1 hybrid mice were generated by intercrossing mouse strains. Mice were housed in specific pathogen–free conditions at the University of Texas Southwestern Medical Center and all experimental procedures were performed in accordance with institutionally approved protocols.

Misawa T., SoRelle J.A., Choi J.H., Yue T., Wang K.W., McAlpine W., Wang J., Liu A., Tabeta K., Turer E., Evers B., Nair-Gill E., Poddar S., Su L., Ou F., Yu L., Russell J., Ludwig S., Zhan X., Hildebrand S., Li X., Tang M., Murray A.R., Moresco E.M, & Beutler B. (2020). Mutual inhibition between Prkd2 and Bcl6 controls T follicular helper cell differentiation. Science immunology, 5(43), eaaz0085.

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Mouse Model for Lupus Nephritis

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NZB x NZW F1 (NZB/W F1) animals were generated by breeding New Zealand White (NZW/LacJ, Stock No: 001058, The Jackson Laboratory) with New Zealand Black (NZB/BINJ, Stock No: 000684, The Jackson Laboratory) animals. Wild-type (WT) female mice were of various genetic backgrounds (Balb/cJ, 129S1, or C57BL/6). WT and NZB/W F1 animals were between 3–12 months of age, depending on the disease stage. All animal experiments were approved by the University of Pennsylvania Institutional Animal Care and Use Committee (IACUC), and euthanasia was performed using carbon dioxide directly prior to spleen isolation.

Syrett C.M., Sierra I., Beethem Z.T., Dubin A.H, & Anguera M.C. (2019). Loss of epigenetic modifications on the inactive X chromosome and sex-biased gene expression profiles in B cells from NZB/W F1 mice with lupus-like disease. Journal of autoimmunity, 107, 102357.

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Inbred Mouse Strain Liver Dissection

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The subjects were adult (10–13 weeks at time of liver dissection), male mice of eight inbred mouse strains: 129S2/SvPasCrl, 129S4/SvJaeJ, 129S8/SvEvNimrJ, BTBR T+ Itpr3tf/J, C57BL/6J, MA/MyJ, NZB/BINJ and SM/J (n = 9 per treatment group per strain, all strains aside from 129S2/SvPasCrl purchased from Jackson Laboratory, Bar Harbor, ME, USA; 129S2/SvPasCrl purchased from Charles River, Wilmington, MA, USA). All mice were group-housed in the same colony room with a 12 h light/dark cycle and ad libitum access to food and water. All procedures were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the Pennsylvania State University IACUC committee.

Zeid D., Mooney-Leber S., Seemiller L.R., Goldberg L.R, & Gould T.J. (2021). Terc Gene Cluster Variants Predict Liver Telomere Length in Mice. Cells, 10(10), 2623.

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