Au5800 automatic biochemistry analyzer

Manufactured by Beckman Coulter

Sourced in United States, China

The AU5800 is an automatic biochemistry analyzer designed to perform a wide range of clinical chemistry tests. It features a high-throughput sample processing system and advanced analytical technologies to deliver accurate and reliable results.

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Lab products found in correlation

Cobas 6000 analyzer series, by Roche (2 mentions) Automated hematology analyzer, by Beckman Coulter (1 mentions) Model 7600 series automatic analyzer, by Hitachi (1 mentions) Uquant microplate reader, by Agilent Technologies (1 mentions)

9 protocols using au5800 automatic biochemistry analyzer

Quantitative Spectrophotometric Assay of G6PD Activity

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G6PD enzyme activity was measured by a quantitative spectrophotometric assay based on the evaluation of absorbance at 340 nm given by NADPH formation. Whole blood samples were collected into tubes containing EDTA-K2. The haemogram analysis was done using an automated hematology analyzer (Beckman Coulter, USA). After purified erythrocytes were lysed, the G6PD activity was determined by commercially available kits (Guangzhou Kofa Biotechnology, Guangdong, China) on Hitachi Model 7600 Series Automatic Analyzer (Hitachi High-Technologies Corporation, Ibaraki, Japan) and AU5800 automatic biochemistry analyzer (Beckman Coulter, USA). The operations were followed by the manufacturer’s instructions. The cutoff value for G6PD deficiency was set at 10.0 IU/g Hb according to the manufacturer’s recommendation. All samples were assayed within 24 h after sample collection to prevent the reduction of G6PD activity. Samples in the presence of obvious hemolysis, abnormal erythrocyte count were excluded^{39 (link)}. G6PD activity was finally expressed as the ratio of G6PD units per gram of Hb.

He Y., Zhang Y., Chen X., Wang Q., Ling L, & Xu Y. (2020). Glucose-6-phosphate dehydrogenase deficiency in the Han Chinese population: molecular characterization and genotype–phenotype association throughout an activity distribution. Scientific Reports, 10, 17106.

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Serum Lipid Quantification Protocol

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Serum lipids including total cholesterol (TC), triacylglycerol (TG), non-esterified fatty acid (NEFA), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were quantified using the Beckman Coulter AU5800 Automatic Biochemistry Analyzer (Beckman Coulter, Shanghai, China).

Li C., Su Z., Chen Z., Cao J., Liu X, & Xu F. (2023). Lactobacillus reuteri strain 8008 attenuated the aggravation of depressive-like behavior induced by CUMS in high-fat diet-fed mice through regulating the gut microbiota. Frontiers in Pharmacology, 14, 1149185.

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Gos Effects on Plasma Potassium in Rabbits

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To study the changes in the plasma K⁺ level caused by Gos, the male rabbits (2.1–2.3 kg) were randomly allocated into three groups, six rabbits in each. The animals were treated with the optimized Gos-GFTs, plain Gos tablets, or Gos drug powder via oral administration, at the dose of 12.5 mg Gos/kg, once every two days. The day of the first administration was marked as day 1. The last administration was on day 39. On day 0 (i.e. the day before the first administration), 10, 20, 30, and 40, the body weight and plasma K⁺ level of each animal were recorded. To determine the plasma K⁺ concentration, a blood sample (about 1.5 mL) of each rabbit was collected via the cardiac apex, followed by centrifugation (5000 rpm × 10 min) to obtain the plasma (Huang et al., 2016 (link)). The concentration of plasma K⁺ was measured using an AU5800 automatic biochemistry analyzer (Beckman Coulter, Inc., USA) with the matched reagent and calibration solution provided by the company (Albert et al., 2011 (link), Woolford et al., 2020 (link)). The measurements were done according to the standard protocol of the biochemistry analyzer.

Liu H., Wang S., Shi H., Zhang R., Qu K., Hu Y., Qu X., Gan C., Chen J., Shi X., Zhang M, & Zeng W. (2021). Gastric floating tablet improves the bioavailability and reduces the hypokalemia effect of gossypol in vivo. Saudi Pharmaceutical Journal : SPJ, 29(4), 305-314.

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Biomarkers of Kidney Injury

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The levels of creatinine (Cre) and blood urea nitrogen (BUN) in serum were measured using an AU 5800 automatic biochemistry analyzer (Beckman Coulter, Inc., USA) according to the manufacturer’s instructions. The concentrations of TNF-α and IL-10 in serum and neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (Kim-1) in urine were measured by ELISA kits (Wuhan Boshi Biotechnology Co., Wuhan, Hubei Province, China) according to the manufacturer’s instructions.

Zhang Z., Zhao H., Ge D., Wang S, & Qi B. (2019). β-Casomorphin-7 Ameliorates Sepsis-Induced Acute Kidney Injury by Targeting NF-κB Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 121-127.

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Glucose Monitoring and 24-Hour Urinary Protein

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During the experiment, glucose was monitored using One Touch Ultra II system (LifeScan, USA) every four weeks using tail vein blood. The 24 h urine protein (24 h UP) was measured by an AU5800 automatic biochemistry analyzer (Beckman, USA).

Li N., Zhao T., Cao Y., Zhang H., Peng L., Wang Y., Zhou X., Wang Q., Li J., Yan M., Dong X., Zhao H, & Li P. (2021). Tangshen Formula Attenuates Diabetic Kidney Injury by Imparting Anti-pyroptotic Effects via the TXNIP-NLRP3-GSDMD Axis. Frontiers in Pharmacology, 11, 623489.

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Serum Lipid Quantification Protocol

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Serum Biomarker Analysis Protocol

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Blood samples were collected via venipuncture, with participants having fasted overnight for at least 8 h. Whole blood was left to stand at room temperature for 30 min, and serum was then collected following centrifugation at 1200× g for 10 min at 4 °C.
The analyzers were calibrated daily before the analysis of all serum samples according to the manufacturer’s protocol. The routine clinical chemistry panel, including UA, ALP, calcium, and phosphorus, was detected using an AU5800 automatic biochemistry analyzer and its corresponding reagents (Beckman Coulter, Brea, CA, USA), with intra- and interassay CVs ranging from 0.5% to 4.9%. The special clinical immunology panel, including 25(OH)D, N-MID, P1NP and β-CTX, was measured using a Cobas 6000 analyzer series and its corresponding reagents (Roche, Basel, Switzerland, CH), with intra- and interassay CVs ranging from 0.6% to 4.3%.

Zhao S.L., Wen Z.X., Mo X.Y., Zhang X.Y., Li H.N., Cheung W.H., Fu D., Zhang S.H., Wan Y, & Chen B.L. (2022). Bone-Metabolism-Related Serum microRNAs to Diagnose Osteoporosis in Middle-Aged and Elderly Women. Diagnostics, 12(11), 2872.

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Serum Biomarker Quantification Protocol

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Blood samples were obtained in the morning via the antecubital vein of participants (after fasting for ≥8 h) and were sent to the department laboratory within 3 hours for postprocessing. The analyzers were calibrated daily before the analysis of all serum samples using quality control standards provided by the manufacturers. Quantitative analysis of serum UA, ALP, Calcium, Phosphorus, Magnesium, ACP, NACP, and PACP was carried out using an AU5800 automatic biochemistry analyzer and its corresponding reagents (Beckman Coulter, Pasadena, CA), with intra- and inter-assay CVs ranging from 0.5% to 4.9%. The levels of serum 25(OH)D, N-MID, P1NP, and β-CTX were measured using a Cobas 6000 analyzer series and its corresponding reagents (Roche, Basel, Switzerland), with intra- and inter-assay CVs ranging from 0.6% to 4.3%.

Zhao S., Mo X., Wen Z., Liu M., Chen Z., Lin W., Huang Z, & Chen B. (2022). Declining serum bone turnover markers are associated with the short-term positive change of lumbar spine bone mineral density in postmenopausal women. Menopause (New York, N.y.), 29(3), 335-343.

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Kidney Function Biomarkers Assessment

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Wet weights of the kidneys removed at necropsy were measured and kidney:body weight ratios calculated. At each timepoint (24 and 72 h after final challenge), blood from the orbital venous plexus was collected into non-anticoagulant-coated tubes, held at 4 C for 2 h, and then centrifuged at 3000 rpm for 15 min. The serum generated was collected and concentrations of serum urea nitrogen (BUN) and creatinine (Cr) then measured in an AU5800 automatic biochemistry analyzer (Beckman Coulter, Brea, CA). Serum a1 MG and b2 MG were assayed using a commercial ELISA kit, following manufacturer instructions. The optical density (OD) in the kit wells was read at 450 nm in a uQUANT microplate reader (BioTek, Winooski, VT). The level of sensitivity of the kit was 0.08 ng a1 MG/ml and 0.02 ng b 2 MG/ml.

Yang L., Zhang J., Li N., Xie H., Chen S., Wang H., Shen T, & Zhu Q.X. (2018). Bradykinin receptor in immune-mediated renal tubular injury in trichloroethylene-sensitized mice: Impact on NF-κB signaling pathway. Journal of immunotoxicology, 15(1).

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