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Edta free cocktail inhibitor tablets

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EDTA-free cocktail inhibitor tablets are a laboratory reagent designed to inhibit a broad range of proteases without the presence of EDTA. They are intended for use in protein sample preparation to preserve the integrity of target proteins.

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Lab products found in correlation

Col10a1, by Thermo Fisher Scientific (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Secondary fluorescent antibodies, by Abcam (2 mentions) Rock inhibitor y2763, by Merck Group (2 mentions) Tead1, by Santa Cruz Biotechnology (2 mentions) Pcdna3, by Addgene (2 mentions) Shyap1 2, by Addgene (2 mentions) Gna13, by Santa Cruz Biotechnology (2 mentions) Rgs12 antibody ab1, by Merck Group (2 mentions) Pdz domain peptides of rgs12, by GenScript (2 mentions) Gpcr activator lpa, by Merck Group (2 mentions) Gpcr inhibitor ki6425, by Merck Group (2 mentions) Rho gtpases inhibitor c3, by Merck Group (2 mentions) Tead1 sirna and controls, by Santa Cruz Biotechnology (2 mentions) Pcdna3.1 gfp yap, by Addgene (2 mentions) Pet gst yap, by Addgene (2 mentions) Pcdna3.1 rhoa, by Addgene (2 mentions) Shrgs12 1, by Applied Biological Materials (2 mentions) Shrgs12 2, by Applied Biological Materials (2 mentions) Shrgs12 3, by Applied Biological Materials (2 mentions)

Close spelling variants of this product

Cocktail inhibitor

4 protocols using edta free cocktail inhibitor tablets

1

Trp53, LAST1 and TAZ Regulation

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Antibodies against Trp53 (#2524; dilution 1:1000), LAST1 (#3477; dilution 1:1000), pLAST1 (#8654; dilution 1:1000), TAZ (#83669; dilution 1:1000), pTAZ (#59971; dilution 1:1000), HA (#5017; dilution 1:1000), GAPDH (#5174; dilution 1:1000); cycloheximide (CHX) and insulin-transferrin-sodium selenite media supplement (ITS Supplement) were purchased from Cell Signaling Technology. BrdU (#sc-32323; dilution 1:1000), β-TrCP (#sc-390629; dilution 1:1000), GFP (#sc-9996; dilution 1:1000) and flag (#sc-7945; dilution 1:1000) antibodies were obtained from Santa Cruz Biotechnology. Col10a1 (#14-9771-80; dilution 1:1000) antibody, EDTA-free cocktail inhibitor tablets, calcein and BrdU labeling were obtained from Fisher Scientific™. The Transfection Reagent FuGENE® HD was ordered from Promega (USA). The plasmids GFP-Trp53, flag-TAZ, and HA-Ub were from Addgene (USA).
Li Y., Yang S.T, & Yang S. (2022). Trp53 controls chondrogenesis and endochondral ossification by negative regulation of TAZ activity and stability via β-TrCP-mediated ubiquitination. Cell Death Discovery, 8, 317.
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2

Antibody-based Analysis of Stem Cell Markers

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Antibodies from Cell Signaling Technology used in this study were against TAZ (#83669; dilution 1:1000), Runx2 (#12556; dilution 1:1000), and GAPDH (#5174; dilution 1:1000). BrdU (#sc-32323; dilution 1:1000), flag (#sc-7945; dilution 1:1000), and GFP (#sc-9996; dilution 1:1000) antibodies were obtained from Santa Cruz Biotechnology. Sox5 (#PIPA5110416; dilution 1:1000) and Col10a1 (#14-9771-80; dilution 1:1000) antibodies were purchased from Fisher Scientific™. The fluorescent secondary antibodies used were mouse and rabbit Alexa Fluor 594 and Alexa Fluor 647, all raised in goat and obtained from Abcam. TEAD1 siRNA was purchased from Santa Cruz Biotechnology. CHX was obtained from Sigma-Aldrich. BrdU labeling, calcein labeling, and EDTA-free cocktail inhibitor tablets, together with the other regents, were obtained from Fisher Scientific™.
Li Y., Yang S., Qin L, & Yang S. (2021). TAZ is required for chondrogenesis and skeletal development. Cell Discovery, 7, 26.
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3

Dissecting YAP/TEAD Signaling Pathway

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Antibodies against p127YAP (D9W2I), YAP (D8H1X), MST1, pMST (E7UD1), LATS1 (C66B5), pLATS1, Lamin B1 (D9V6H) and GAPDH were from CST. Antibodies of Ezrin, GNA12, GNA13, RhoA, flag, TEAD1, GST and GFP were from Santa Cruz Biotechnology. RGS12 antibody (ab1) was from Sigma. The secondary fluorescent antibodies were from Abcam. PDZ domain peptides of RGS12 were purchased from GenScript. GPCR activator LPA, GPCR inhibitor Ki6425, Rho GTPases inhibitor C3 and Rock inhibitor ‎Y2763 were obtained from Sigma. Doxorubicin hydrochloride (DOX), methotrexate 4-Amino-10-methylfolic acid hydrate (MTX) and EDTA-free cocktail inhibitor tablets were all obtained from Fisher Scientific™. TEAD1 siRNA and controls were from Santa Cruz Biotechnology. FuGENE® HD Transfection Reagent was purchased from Promega Corporation. The primers used for the quantification are listed in Supplementary Table 1.
The following plasmids: pRL-TK was generously provided by Dr. Zhen Zhang (University of Pennsylvania, Philadelphia, PA, USA); pcDNA3.1, shYAP1/2, pcDNA3.1-GFP-YAP, PET-GST-YAP and pcDNA3.1-RhoA were obtained from Addgene. Three human shRGS12 lentivectors (shRGS12–1, shRGS12–2 and shRGS12–3; Catalog # i019000) were ordered from ABM. pcDNA3.1-flag-RGS12 (flag-RGS12) and the pcDNA3.1-flag-RGS12 mutant with the deletion of the PDZ domain vectors (flag-RGS12ΔPDZ) were constructed in our lab.
Li Y., Liu M., Yang S., Fuller A.M., Eisinger T.S, & Yang S. (2021). RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling. Oncogene, 40(14), 2553-2566.
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4

Dissecting YAP/TEAD Signaling Pathway

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Antibodies against p127YAP (D9W2I), YAP (D8H1X), MST1, pMST (E7UD1), LATS1 (C66B5), pLATS1, Lamin B1 (D9V6H) and GAPDH were from CST. Antibodies of Ezrin, GNA12, GNA13, RhoA, flag, TEAD1, GST and GFP were from Santa Cruz Biotechnology. RGS12 antibody (ab1) was from Sigma. The secondary fluorescent antibodies were from Abcam. PDZ domain peptides of RGS12 were purchased from GenScript. GPCR activator LPA, GPCR inhibitor Ki6425, Rho GTPases inhibitor C3 and Rock inhibitor ‎Y2763 were obtained from Sigma. Doxorubicin hydrochloride (DOX), methotrexate 4-Amino-10-methylfolic acid hydrate (MTX) and EDTA-free cocktail inhibitor tablets were all obtained from Fisher Scientific™. TEAD1 siRNA and controls were from Santa Cruz Biotechnology. FuGENE® HD Transfection Reagent was purchased from Promega Corporation. The primers used for the quantification are listed in Supplementary Table 1.
The following plasmids: pRL-TK was generously provided by Dr. Zhen Zhang (University of Pennsylvania, Philadelphia, PA, USA); pcDNA3.1, shYAP1/2, pcDNA3.1-GFP-YAP, PET-GST-YAP and pcDNA3.1-RhoA were obtained from Addgene. Three human shRGS12 lentivectors (shRGS12–1, shRGS12–2 and shRGS12–3; Catalog # i019000) were ordered from ABM. pcDNA3.1-flag-RGS12 (flag-RGS12) and the pcDNA3.1-flag-RGS12 mutant with the deletion of the PDZ domain vectors (flag-RGS12ΔPDZ) were constructed in our lab.
Li Y., Liu M., Yang S., Fuller A.M., Eisinger T.S, & Yang S. (2021). RGS12 is a novel tumor suppressor in osteosarcoma that inhibits YAP-TEAD1-Ezrin signaling. Oncogene, 40(14), 2553-2566.
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