Image pro plus

Manufactured by Zeiss

Sourced in United States, Germany

Image-Pro Plus is a comprehensive image analysis software developed by Zeiss. It provides advanced tools for processing, analyzing, and quantifying digital images. The software supports a wide range of image file formats and offers a suite of features for tasks such as measurement, object detection, and data visualization. Image-Pro Plus is designed to be a versatile tool for researchers and professionals working with digital imaging applications.

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Lab products found in correlation

Rabbit anti periostin antibody, by Abcam (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) Sp1600, by Leica (1 mentions) Aperio at2, by Leica (1 mentions) Inverted microscope, by Zeiss (1 mentions) Axio observer microscope, by Zeiss (1 mentions) 24 wells, by Corning (1 mentions) 710 confocal microscope, by Zeiss (1 mentions) Anti c fos, by Santa Cruz Biotechnology (1 mentions) Anti quenching mounting medium, by Thermo Fisher Scientific (1 mentions) Fluorescence conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Lsm 510 laser confocal fluorescence microscope, by Zeiss (1 mentions) Anti β1 ar, by Santa Cruz Biotechnology (1 mentions) Chicken anti nestin, by Novus Biologicals (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) 710 meta confocal microscope, by Zeiss (1 mentions) 35 mm glass bottom dishes, by MatTek (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Alexa flour 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Image-Pro Plus 6.0 (31 citations) Image-Pro Plus software (7 citations) Image-Pro® Plus v 6.0 (6 citations) Image-Pro (3 citations) Image Pro Plus 7.0 (2 citations) Image-Pro Plus 3.0 (2 citations)

11 protocols using image pro plus

Histological Evaluation of Dental Implant Osseointegration

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The specimens euthanized after 7 days were decalcified in 10% ethylenediaminetetraacetic acid (EDTA) for 45 days and embedded in paraffin. The implants were unscrewed and 5‑μm‑thick sections were prepared and stained with hematoxylin and eosin (H&E). For immunohistochemistry, sections were incubated with rabbit anti‑CD63 antibody (1:100; Abclonal), rabbit anti‑periostin antibody (1:1000; Abcam) and rabbit anti‑CD11b antibody (1:4000; Abcam) overnight at 4 ℃ followed by incubation with a horseradish peroxidase (HRP)‑conjugated secondary antibody. Sections were examined with a digital pathology scanner (Aperio AT2; Leica Biosystems, USA) and evaluated by calculating the mean optical density (MOD) of CD63, periostin and CD11b expression using Image-Pro Plus 6.0 (Media Cybernetics, USA).
The specimens euthanized after 28 days were dehydrated in a gradient series of ethanol and embedded in polymethylmethacrylate. Sections with a final thickness of 50 μm were prepared using a saw (Leica SP1600; Leica Biosystems) and stained with methylene blue acid solution. Digital images at 25× magnification were acquired with an Axio Imager.Z2 (Zeiss) and analyzed for the bone-implant contact (BIC) ratio with Image-Pro Plus.

Zhang Z., Xu R., Yang Y., Liang C., Yu X., Liu Y., Wang T., Yu Y, & Deng F. (2021). Micro/nano-textured hierarchical titanium topography promotes exosome biogenesis and secretion to improve osseointegration. Journal of Nanobiotechnology, 19, 78.

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Wound Closure Assay with TNF-α

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MDA-MB-231 and MCF7 cells were seeded in Petri dishes and cultured to reach 90 % confluency in complete medium. Next, the cells were starved overnight and a sterile 200 μl pipet tip was used to scrape four wounds through the cell monolayer. The cells were gently rinsed before being treated with 10 ng/ml TNF-α. Pictures were taken immediately before TNF-α application and after 24 h using phase contrast optics. Images were acquired with a 10×/1.25 numerical aperture objective on an inverted microscope (Zeiss) equipped with a digital camera using Image-Pro Plus version 5.1.2.59. Wound areas were quantified using imageJ software version 1.43u. The results are presented as wound closure rates.

Wolczyk D., Zaremba-Czogalla M., Hryniewicz-Jankowska A., Tabola R., Grabowski K., Sikorski A.F, & Augoff K. (2016). TNF-α promotes breast cancer cell migration and enhances the concentration of membrane-associated proteases in lipid rafts. Cellular Oncology (Dordrecht), 39, 353-363.

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Quantifying Hepatic Neutral Lipids

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Hepatic neutral lipid was detected by a standard Oil Red O staining. Frozen liver tissue sections fixed in 4% formalin were warmed to room temperature. After washing with PBS, the sections were incubated in 100% propylene glycol for 10 min and then stained with 0.7% Oil Red O in propylene glycol. The Oil Red O dye was then washed off with 85% propylene glycol. Images of the sections were captured with a Zeiss microscope and analyzed using Image-Pro Plus.

Park J.H., Chung H.Y., Kim M., Lee J.H., Jung M, & Ha H. (2014). Daumone fed late in life improves survival and reduces hepatic inflammation and fibrosis in mice. Aging Cell, 13(4), 709-718.

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Immunofluorescence Analysis of MHC Expression

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For immunofluorescence analysis, after differentiation, cells were washed with cold PBS and then fixed in 4% paraformaldehyde for 40 minutes and permeabilized in 0.2% Triton X-100-PBS for 10 minutes. After washing three times (10 minutes each) in PBS, cells were blocked with 5% BSA for 2 hours at room temperature and then incubated with anti-MHC primary antibody (Santa Cruz) at 4°C overnight. Alexa Fluor 488-labeled goat anti-mice IgG (Beyotime, China) was used as secondary antibody, and nuclei were stained with DAPI. The fluorescence images were gathered using Axio Observer microscope (Zeiss, USA) and Image Pro Plus.

Liu X., Wang Y., Zhao S, & Li X. (2017). Fibroblast Growth Factor 21 Promotes C2C12 Cells Myogenic Differentiation by Enhancing Cell Cycle Exit. BioMed Research International, 2017, 1648715.

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Quantifying Airway Epithelial Volume and Mucosubstances

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Mucosubstances and airway epithelial volume were evaluated by measuring intracellular mucosubstances and overall airway epithelial volume in sections stained with alcian blue/periodic acid-Schiff (AB/PAS) (American Master Tech Scientific) and H&E, respectively. Four fields were sampled in the main axial airway path with light microscopy (Carl Zeiss Microscopy) and an image-analysis system (Image-Pro Plus). Images were overlaid with a cycloid arc grid in Image J (NIH) software to measure the volume of mucin volume per surface area of epithelial basal lamina. This was done to assess the volume fraction of epithelial AB/PAS staining, which was defined by total points hitting the objectives and total points falling within the reference space. In addition, epithelial volume was assessed using the same images and is expressed as the volume of epithelium per surface area of epithelial basal lamina. The airway epithelial volume fraction was also measured at the level of the proximal region of the main axial airway path. This region constitutes the 2^nd and 3^rd lobar airway generations of the left lung.

Pham A.K., Wu C.W., Qiu X., Xu J., Smiley-Jewell S., Uyeminami D., Upadhyay P., Zhao D, & Pinkerton K.E. (2020). Differential lung inflammation and injury with tobacco smoke exposure in Wistar Kyoto and Spontaneously Hypertensive rats. Inhalation toxicology, 32(8), 328-341.

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Osteoclast Resorption Assay Protocol

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The function of osteoclasts was assessed by the resorption assay. Briefly, BMMs were inoculated in osteo assay surface plates containing 24-wells (Corning, USA) at a concentration of 10 × 10⁴/well. Then groups of BMMs were induced to differentiate toward osteoclasts as before. After 6 days, trypsin digestion and three PBS washes were performed on the cells. Following that, images were captured with a simple light microscope (Zeiss), and Image Pro-Plus (Version-6.0) was employed to analyze the region of the resorption depression.

Jiao Z., Chai H., Wang S., Sun C., Huang Q, & Xu W. (2023). SOST gene suppression stimulates osteocyte Wnt/β-catenin signaling to prevent bone resorption and attenuates particle-induced osteolysis. Journal of Molecular Medicine (Berlin, Germany), 101(5), 607-620.

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Stripe-patterned Assay for NPC Migration

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A silicon wafer was used to generate a template for the PDMS mold and substrates were patterned in parallel stripes of 60 μm width separated by 90 μm gaps. Briefly, PDMS mold was reversibly sealed on PDL-coated glass bottom dishes (35 mm dish with 20 mm bottom well), and microchannels were formed between the PDMS mold and well. The mixture of BSA and CXCL12 or BSA alone was added to one end of the microchannels, and vacuum was applied to the other end of the microchannels to ensure BSA/CXCL12 or BSA filled in all the microchannels. PDMS molds were removed after drying overnight. The stripe-coated dishes were planted with dissociated NPCs to study cell migration or polarization. Cells were then fixed on cover glasses and stained with Nestin and DAPI. Images were taken by a Zeiss 710 confocal microscope and quantified by Image Pro Plus. The percent of cells on stripe versus total cell number was calculated to evaluate cell migration.

Zhang M., Song A., Lai S., Qiu L., Huang Y., Chen Q., Zhu B., Xu D, & Zheng J.C. (2015). Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization. Biomaterials, 72, 163-171.

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Brain Perfusion and Immunofluorescence Imaging

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The mice were perfused intracardiacally with saline first, then with 4% paraformaldehyde in 0.1 M Na₂HPO₄/NaH₂PO₄ buffer (pH 7.5) and the brains were removed. After post-fixation in 4% paraformaldehyde for 4 hours, the samples were stored in 30% sucrose/PBS for 3 days. Brain slices 30 μm thick were incubated in primary antibody (anti-c-Fos 1: 1000, Santa Cruz; anti-β1-AR 1:100, Santa Cruz) at 4°C overnight. After being washed with PBS 3 times, the slices were incubated with fluorescence conjugated secondary antibody at room temperature (1:50000, Jackson ImmunoResearch) for 1 hour. Then the brain sections were rinsed in PBS and mounted with antiquenching mounting medium (Thermo Fisher Scientific). The sections were visualized under a LSM 510 laser confocal fluorescence microscope (Carl Zeiss) and analyzed by Image-Pro Plus. Labeled cells above the same threshold determined from control animals were counted (Trifilieff et al., 2006 ).

Zhu H., Zhou Y., Liu Z., Chen X., Li Y., Liu X, & Ma L. (2017). β1-Adrenoceptor in the Central Amygdala Is Required for Unconditioned Stimulus-Induced Drug Memory Reconsolidation. International Journal of Neuropsychopharmacology, 21(3), 267-280.

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Isolation and Characterization of Mouse Forebrain Neural Progenitor Cells

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The forebrain of each mouse embryo at E13.5 was dissected and mechanically dissociated. Cells from each forebrain were seeded into a 100 mm Petri dish at a density of 2 × 10⁵ cells/ml in 10 ml of mouse NeuroCult NSC Proliferation Medium (Stem Cell Technologies) supplemented with epidermal growth factor (20 ng/ml; Gibco) and basic fibroblast growth factor (10 ng/ml; Gibco) for selective neurosphere growth. Neurospheres were passaged when they reached 100–150 μm in diameter. For immunochemistry, mouse NPCs were grown in 35 mm glass-bottom dishes (MatTek) at a density of 8 × 10⁴ cells/ml for 24 h, fixed using 4% PFA, and permeabilized with 0.4% Triton-X in PBS. Subsequently, they were incubated overnight at 4 °C with primary antibodies including mouse anti-Ki67 (1:200; Cell Signaling) and chicken anti-Nestin (1:500; Novus) for the identification of proliferating NPCs. This was followed by incubation with secondary antibodies: goat anti-mouse IgG (conjugated with Alexa Flour 488; Invitrogen) and goat anti-chicken IgG (conjugated with Alexa Fluor 568; Invitrogen). Nuclei were counter-stained with DAPI. Immunostaining was examined by a Zeiss META 710 confocal microscope and images were imported into Image-ProPlus, version 7.0 for qualification.

Xu P., Wang Y., Qin Z., Qiu L., Zhang M., Huang Y, & Zheng J.C. (2017). Combined Medication of Antiretroviral Drugs Tenofovir Disoproxil Fumarate, Emtricitabine, and Raltegravir Reduces Neural Progenitor Cell Proliferation In Vivo and In Vitro. Journal of Neuroimmune Pharmacology, 12(4), 682-692.

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Immunofluorescent Staining of Cells

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Cells were fixed in 4% paraformaldehyde (PFA) for 15 min at 4°C, then permeabilized and blocked in PBS containing 1% BSA, 4% goat serum, and 0.4% Triton X-100 for 1 h at room temperature. Subsequently, cells were incubated with primary antibody (anti-β-tubulin III, 1:1000, Sigma, mouse monoclonal antibody) overnight at 4°C, followed with secondary antibody (anti-mouse, CY3, 1:400, Jackson Immuno Research) for 2 h at room temperature. Images of cells were captured by a Zeiss LSM 710 confocal microscope with a 20 × objective. Images were analyzed with Zen 2012 (Zeiss) and Image-Pro Plus.

Qi Y., Ma N., Chen X., Wang Y., Zhang W, & Wan J. (2021). CircRtn4 Acts as the Sponge of miR-24-3p to Promote Neurite Growth by Regulating CHD5. Frontiers in Molecular Neuroscience, 14, 660429.

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