Plasma was collected from peripheral blood, spun at 3000 g for 2 min and stored at −20°C until assayed. IFN-γ levels throughout infection were quantified using the IFN-γ ‘Femto-HS’ High Sensitivity Mouse Uncoated ELISA Kit (Invitrogen), according to manufacturer instructions. The concentration of pro-inflammatory cytokines at day 6 PI was assessed by cytometric bead array (Legendplex custom multi-analyte flow assay kit, Biolegend) according to the manufacturer’s protocol.
Ifn γ femto hs high sensitivity mouse uncoated elisa kit
Manufactured by Thermo Fisher Scientific
The IFN-γ 'Femto-HS' High Sensitivity Mouse Uncoated ELISA Kit is a laboratory product designed for the quantitative measurement of interferon-gamma (IFN-γ) in mouse samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.
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2 protocols using ifn γ femto hs high sensitivity mouse uncoated elisa kit
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Quantifying Cytokine Levels in Infection
2
Quantifying Cytokine Levels in Infection
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Plasma was collected from peripheral blood, spun at 3000 g for 2 min and stored at −20°C until assayed. IFN-γ levels throughout infection were quantified using the IFN-γ ‘Femto-HS’ High Sensitivity Mouse Uncoated ELISA Kit (Invitrogen), according to manufacturer instructions. The concentration of pro-inflammatory cytokines at day 6 PI was assessed by cytometric bead array (Legendplex custom multi-analyte flow assay kit, Biolegend) according to the manufacturer’s protocol.
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