The protein samples of 6T-CEM cells were loaded (5 μg) on a 10% acrylamide SDS-PAGE gel (Bio-Rad, Hercules, CA, USA) for protein separation, followed by transfer to PVDF membranes (Bio-Rad). The blots were then blocked with 1% BSA in 0.1% Tween 20/PBS for 1 h at room temperature and then incubated overnight at 4°C with antibodies specific for CXCL16 (PeproTech, Rocky Hill, CT). After washing with 0.1% Tween 20 in PBS, the membranes were incubated with a 1 : 3000 dilution of goat anti-rabbit IgG HRP (Calbiochem) in 1% solution of powdered milk in PBS/0.1% Tween 20. The membranes were exposed to ECL solution (Bio-Rad) and imaged by chemiluminescence (Clinx Science Instrument, Shanghai, China).
Acrylamide sds page gel
Manufactured by Bio-Rad
Sourced in United States
10% acrylamide SDS-PAGE gel is a laboratory equipment used for protein separation and analysis through electrophoresis. It consists of a 10% concentration of acrylamide in a sodium dodecyl sulfate (SDS) buffer solution, which facilitates the separation of proteins based on their molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Ecl solution, by Bio-Rad (2 mentions)
Pvdf membrane, by Bio-Rad (2 mentions)
Pser9 gsk3β, by Cell Signaling Technology (2 mentions)
P ser455, by Cell Signaling Technology (2 mentions)
P ser724 ireα, by Cell Signaling Technology (2 mentions)
P ireα, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Goat anti rabbit igg hrp, by Merck Group (1 mentions)
4 protocols using acrylamide sds page gel
1
Western Blot Analysis of CXCL16 Protein
2
Western Blot Analysis of ARRB2 in Mouse Lung
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The protein samples of mouse lung tissue or MLE-12 cells were loaded (5 μg) on a 10% acrylamide SDS-PAGE gel (Bio-Rad, Hercules, USA) for protein separation, followed by transfer to PVDF membranes (Bio-Rad). The blots were then blocked with 1% BSA in 0.1% Tween 20/TBS for 1 h at room temperature and then incubated overnight at 4°C with antibodies specific for ARRB2 (Novus Biologicals, Centennial, CO, USA). After washing with 0.1% Tween 20 in TBS, the membranes were incubated with a 1: 3000 dilution of goat anti-rabbit IgG HRP (EMD Millipore Corp, Burlington, Massachusetts, USA) in 1% solution of powdered milk in TBS/0.1% Tween 20. The membranes were exposed to ECL solution (Bio-Rad) and imaged by chemiluminescence (Clinx Science Instrument, Shanghai, China).
3
Adipose Tissue Protein Analysis
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Adipose tissues were homogenized on ice and protein concentrations were determined by Bradford assay as previously described (18) Adipose proteins were fractionated on 4–15% acrylamide SDS-PAGE gels (BioRad, Hercules, CA, USA) and transferred onto nitrocellulose membranes for the western analysis of p-Ser79 ACC, p-Ser455ACL, p-threonine308 kinase Akt/PKB, IR, IRS-1, GLUT4, p-Ser9 GSK3β, p-Ser724 IREα, ACC, ACL, XBP1, p-IREα, IREα, and GAPDH (Cell Signaling, Danvers MA). Proteins signals were quantified by Eagle Eye II Quantitation System (Stratagene, CA, USA).
4
Adipose Tissue Protein Analysis
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