50i inverted microscope

Manufactured by Nikon

The Nikon 50i inverted microscope is a compact and versatile laboratory instrument designed for a wide range of microscopy applications. It features a stable and ergonomic design, providing a stable platform for accurate observations. The 50i inverted microscope is equipped with a range of optical components, including objectives and eyepieces, allowing for various magnification levels to suit different research and analysis needs.

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3 protocols using 50i inverted microscope

Histological Scoring of Intestinal Inflammation

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For histology, the colons were washed, fixed in 4% buffered formaldehyde, and embedded in paraffin. Tissue sections were stained with H&E. Histology was scored in a blinded fashion as a combination of inflammatory cell infiltrate (score, 0 to 4) and intestinal architecture damage (scores 0 to 4). The presence of occasional inflammatory cells in the LP was scored as 0; increased numbers of inflammatory cells in the LP was scored as 1; inflammatory cells extending into the mucosa and submucosa were scored as 2; inflammatory cells extending into the mucosa, submucosa, and, sometimes, transmural infiltration were scored as 3; and severe transmural extension of the infiltrate was scored as 4. For intestinal architecture damage, no mucosal damage was scored as 0; focal erosions were scored as 1; slight crypt loss and focal ulcerations were scored as 2; extended ulcerations and moderate crypt loss were scored as 3; and extensive crypt loss, mucosal damage, and extension into deeper structures of the bowel wall were scored as 4. The combined histological score ranged from 0 (no changes) to 8 (extensive infiltration and tissue damage). Tissues were stained with PAS for showing goblet cells. Images were acquired with a Nikon 50i inverted microscope.

Ma C., Yang D., Wang B., Wu C., Wu Y., Li S., Liu X., Lassen K., Dai L, & Yang S. (2020). Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation. Science Advances, 6(21), eaaz6717.

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Histological Scoring of Intestinal Inflammation

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For histology, tissue sections were stained with hematoxylin & eosin (H&E). Histology was scored in a blinded fashion as a combination of inflammatory cell infiltration (score 0–4) and intestinal architecture damage (score 0–4). The presence of occasional inflammatory cells in the lamina propria was scored as 0; increased numbers of inflammatory cells in the lamina propria was scored as 1; inflammatory cells extending into the mucosa and submucosa was scored as 2; inflammatory cells extending into the mucosa, submucosa, and sometimes transmural infiltration was scored as 3; and severe transmural extension of the infiltrate was scored as 4. For architecture damage, no mucosal damage was scored as 0; focal erosions were scored as 1; slight crypt loss and focal ulcerations was scored as 2; extended ulcerations and moderate crypt loss was scored as 3; and extensive crypt loss, mucosal damage, and extension into deeper structures of the bowel wall was scored as 4. The total histologic score was derived by summing each individual score. Images were acquired with a Nikon 50i inverted microscope.

Liu L., Wu Y., Wang B., Jiang Y., Lin L., Li X, & Yang S. (2021). DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization. Cell Death & Disease, 12(6), 500.

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Histopathological Assessment of Tissue Injury

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The tissue was fixed in 4% buffered formaldehyde for histology and embedded in paraffin. Tissue sections were stained with H&E. To evaluate the intestinal injury, Chui’s scoring system was used as previously described [15 (link)]. To evaluate lung inflammation and damage, the entire lung surface was analyzed with respect to the following parameters: neutrophils in the alveolar space, neutrophils in the interstitial space, hyaline membranes, proteinaceous debris filling the airspaces, and alveolar septal thickening. Each parameter received a score from 0 (absent) to 2 (severe). Liver inflammation was scored according to the following parameters: number of thrombi, number of (micro)abscesses, presence and degree of inflammation, and presence and degree of necrosis. Each parameter received a score from 0 (absent) to 3 (severe). Images were acquired with a Nikon 50i inverted microscope.

Wu J., Liu Q., Zhang X., Tan M., Li X., Liu P., Wu L., Jiao F., Lin Z., Wu X., Wang X., Zhao Y, & Ren J. (2022). The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages. Cell Death & Disease, 13(7), 653.

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