Anti human igg fab 2 goat monoclonal antibody

The following human recombinant proteins and antibodies were used: human SARS-CoV-2 (2019-nCoV) chimeric Spike RBD-Fc protein (Sino Biological, 10108-H08H, Eschborn, Germany); human recombinant IgG1 Fc protein (R & D Systems, 110-HG, Minneapolis, MN, USA); human Platelet Factor 4 (Creative Biomart, PF4-52H, Shirley, NY, USA).
HRP conjugated anti-human Fc antibody (Sigma, AP113P, St. Louis, MO, USA); anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, ab98535, Cambridge, UK); rabbit polyclonal anti-PF4 antibody (Prodotti Gianni, ab9561, Milano, Italy).

The following human recombinant proteins were used: SARS-CoV-2 (2019-nCoV) chimeric Spike RBD-Fc protein, SARS-CoV-2 (2019-nCoV) Spike RBD-His protein and ACE2 His Protein (all from Sino Biological, 10108-H08H Düsseldorfer Eschborn, Germany); Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (from Abcam, ab273068, Cambridge UK); Recombinant ACE-2 Fc chimeric protein (Z03484 GenScript, Piscataway NJ); recombinant IgG1 Fc protein (R & D Systems, 110-HG Minneapolis MN).
The following antibodies were used: HRP conjugated anti-His antibody (Qiagen, 1014992 Hilden, Germany); HRP conjugated anti-cmyc antibody (Milteny Biotec, 130-092-113 GmbH Friedrich-Ebert-Strabe Germany); HRP conjugated anti-human Fc antibody (Sigma, AP113P USA); anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, Cambridge UK ab98535).

The following antibodies were used: anti-CTLA-4 mAb Ipilimumab (Yervoy, Bristol Myers Squibb, NY, USA); commercial Human CTLA-4 Antibody (R & D Systems, Minneapolis, MN, USA); anti-phospho44/42 MAPK (indicated as pErk); anti-phospho-(Ser/Thr) Akt; anti-Cleaved Caspase-3 polyclonal antibody antibodies (all from Cell Signaling Technology, Danvers, MA, USA); anti-vinculin; anti-p-Tyr polyclonal antibodies (both from Santa Cruz Biotechnology, Inc. Dallas, Texas USA; anti-actin antibody (Sigma-Aldrich, Darmdstadt, Germany); anti-His HRP conjugated mAb (Qiagen, Hilden, Germany); anti-human IgG (H+L) HRP conjugate antibody (Promega, Madison, WI, USA); anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, Cambridge, UK, ab98535).
The following recombinant proteins were used: Recombinant Human CTLA-4 Fc Chimera; Recombinant Human B7-1/CD80 Fc Chimera Protein; Recombinant Human B7-2/CD86 Fc Chimera Protein; Recombinant Human CD28 Fc protein; Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein; Recombinant Human IgG1 Fc Protein (all from R & D Systems, Minneapolis, MN, USA); Human CTLA-4 Protein (His & Fc Tag); CTLA-4 Protein, Mouse, Recombinant (Fc Tag) (both from Sino Biological, Wayne, PA, USA).