Nonoxidized cello oligosaccharides

Mono-, oligosaccharides and their corresponding aldonic acid and C4-gemdiol forms generated after PASC cleavage were analyzed by high-performance anion exchange chromatography coupled with amperometric detection (HPAEC-PAD) as described by Westereng et al. [37 (link)] using nonoxidized cello-oligosaccharides (Megazyme, Wicklow, Ireland) as standards. Oligosaccharides standards oxidized at the C1 position were produced from nonoxidized cello-oligosaccharides using a cellobiose dehydrogenase as described in Bennati-Granier C. et al. [15 (link)]. For analysis of xyloglucan degradation products, purified xylo-oligosaccharides of known structure XXXG, XXLG, and XLLG according to the nomenclature of Fry et al. [38 (link)] were used as standards (Megazyme reference O-XGHON).

The oxidized and non-oxidized oligosaccharides generated after action of PaLPMO9E on bleached birchwood Kraft fibers were analyzed by high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) (Thermo Fisher Scientific, Waltham, USA) as described by Westereng et al. [52 (link)] using non-oxidized cello-oligosaccharides as standards (Megazyme, Wicklow, Ireland). Oligosaccharides standards oxidized at the C1 position were produced from non-oxidized cello-oligosaccharides using a cellobiose dehydrogenase as described in Bennati-Granier et al. (31).