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Rrgfr α3

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RrGFR-α3 is a recombinant protein that functions as the receptor for glial cell-derived neurotrophic factor (GDNF). It is a member of the GDNF receptor alpha (GFRα) family and plays a role in the signaling of GDNF.

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Lab products found in correlation

Sb202190, by Merck Group (2 mentions) Pstat3, by Merck Group (2 mentions) P38 mapk, by Cell Signaling Technology (2 mentions) Pd98059, by Merck Group (2 mentions) Stat3, by BD (2 mentions) P38 mapk, by Merck Group (2 mentions) Rrgfr α1, by R&D Systems (2 mentions) Rmgfr α2, by R&D Systems (2 mentions) Rrgndf, by R&D Systems (2 mentions) Rhnrtn, by Thermo Fisher Scientific (2 mentions) Rhartn, by Thermo Fisher Scientific (2 mentions) P38 mapk erk akt ly294002 ly, by Merck Group (2 mentions)

2 protocols using rrgfr α3

1

Regulation of ILC3 cell signaling pathways

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Sorted intestinal ILC3 cells were starved for 3 hours in RPMI at 37°C in order to ensure ILC3 viability. Retfl or RetΔ were analysed directly ex vivo. To test ERK, AKT, p38-MAPK (Cell Signaling Technology) and STAT3 (BD Pharmigen) upon GFL stimulation WT ILC3 were activated with 500ng/mL (each GFL) and co-receptors (rrGFR-α1, rmGFR-α2, rrGFR-α3 and rrGNDF from R&D Systems; rhNRTN and rhARTN from PeproTech) for 10 and 30min. When referring to the use of ‘GFL’, we have employed GDNF, NRTN, ARTN and their specific co-receptors in combination. For inhibition experiments cells were incubated 1h at 37°C before GFL stimulation, to test ERK, AKT, p38/MAPK and STAT3 phosphorylation, or during overnight stimulation with GFLs, to determine Il22 expression levels. Inhibitors were purchased from Sigma-Aldrich: p38 MAPK/ERK-AKT - LY294002 (LY); ERK - PD98059 (PD); AKT - AKT Inhibitor VIII (VIII); p38 MAPK - SB 202190 (SB); and pSTAT3 – S3I-201 (S3I).
Ibiza S., García-Cassani B., Ribeiro H., Carvalho T., Almeida L., Marques R., Misic A.M., Bartow-McKenney C., Larson D.M., Pavan W.J., Eberl G., Grice E.A, & Veiga-Fernandes H. (2016). Glial cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature, 535(7612), 440-443.
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2

Regulation of ILC3 cell signaling pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted intestinal ILC3 cells were starved for 3 hours in RPMI at 37°C in order to ensure ILC3 viability. Retfl or RetΔ were analysed directly ex vivo. To test ERK, AKT, p38-MAPK (Cell Signaling Technology) and STAT3 (BD Pharmigen) upon GFL stimulation WT ILC3 were activated with 500ng/mL (each GFL) and co-receptors (rrGFR-α1, rmGFR-α2, rrGFR-α3 and rrGNDF from R&D Systems; rhNRTN and rhARTN from PeproTech) for 10 and 30min. When referring to the use of ‘GFL’, we have employed GDNF, NRTN, ARTN and their specific co-receptors in combination. For inhibition experiments cells were incubated 1h at 37°C before GFL stimulation, to test ERK, AKT, p38/MAPK and STAT3 phosphorylation, or during overnight stimulation with GFLs, to determine Il22 expression levels. Inhibitors were purchased from Sigma-Aldrich: p38 MAPK/ERK-AKT - LY294002 (LY); ERK - PD98059 (PD); AKT - AKT Inhibitor VIII (VIII); p38 MAPK - SB 202190 (SB); and pSTAT3 – S3I-201 (S3I).
Ibiza S., García-Cassani B., Ribeiro H., Carvalho T., Almeida L., Marques R., Misic A.M., Bartow-McKenney C., Larson D.M., Pavan W.J., Eberl G., Grice E.A, & Veiga-Fernandes H. (2016). Glial cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature, 535(7612), 440-443.
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