Ab40810

Manufactured by Abcam

Ab40810 is a lab equipment product. It is a monoclonal antibody that targets a specific protein. The core function of this product is to detect and bind to the target protein for research and analysis purposes.

Automatically generated - may contain errors

Lab products found in correlation

Ab88078, by Abcam (1 mentions) Enhanced chemiluminescence, by Cytiva (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions) Actin tracker red rhodamine, by Beyotime (1 mentions) Nb100 125, by Novus Biologicals (1 mentions) Sc 8008, by Santa Cruz Biotechnology (1 mentions) Ab16502, by Abcam (1 mentions)

2 protocols using ab40810

Protein Expression Analysis of Hippocampal Tissue

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The powdered hippocampal tissues were homogenized in ice-cold lysis buffer. Aliquots of the clarified homogenized liquid containing 75 μg of protein were denatured and analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Bio-Rad). The primary antibodies included rabbit monoclonal anti-Gys 1 (1:2000, Abcam, ab40810), mouse monoclonal anti-Gyp (1:2000, Abcam, ab88078), and mouse monoclonal anti-β-actin (1:2000, Sigma, A1978). The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:4000, GeneScript) or goat anti-rabbit IgG (1:4000, GeneScript). Immunoblotting was detected by enhanced chemiluminescence (Amersham) and analyzed using an FR-200A Electrophoresis Image Analysis System (Furi, Shanghai, China). The values of Gys 1 and Gyp levels were normalized against the amount of β-actin obtained from the same sample.

Zhao Y., Zhang Q., Shao X., Ouyang L., Wang X., Zhu K, & Chen L. (2017). Decreased Glycogen Content Might Contribute to Chronic Stress-Induced Atrophy of Hippocampal Astrocyte volume and Depression-like Behavior in Rats. Scientific Reports, 7, 43192.

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Antibody Panel for Metabolic Regulation

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The following antibodies were used in this study: anti-GYS1 (ab40810, abcam), anti-PER2 (NB100-125, Novus Bio), anti-PER2 (sc377290, Santa Cruz), anti-NFκB p65 (sc-8008, Santa Cruz), anti-NFκB p65 (ab16502, abcam), and anti-Flag (#14793, CST). ELISA kit for mouse was purchased from eBioscience. Actin-Tracker Red-Rhodamine (C2207S) was purchased from Beyotime. Per2 shRNA lentiviral particles were purchased from Genepharma.

Ding Z., Ge W., Xu X., Xu X., Wang S, & Zhang J. (2023). PER2/P65-driven glycogen synthase 1 transcription in macrophages modulates gut inflammation and pathogenesis of rectal prolapse. The Journal of Biological Chemistry, 299(10), 105219.

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