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Leptin immunoassay kit

Manufactured by R&D Systems
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The Leptin Immunoassay Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of leptin levels in biological samples. The kit utilizes a monoclonal antibody specific for leptin that is pre-coated onto a microplate. Samples and standards are pipetted into the wells, and any leptin present is bound by the immobilized antibody. After washing, an enzyme-linked polyclonal antibody specific for leptin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of leptin bound. The intensity of the color is measured.

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Lab products found in correlation

Transaminase cii test wako kit, by Fujifilm (1 mentions) Mouse insulin elisa kit, by Mercodia (1 mentions)

Close spelling variants of this product

Quantikine ELISA mouse Leptin Immunoassay kit Mouse leptin immunoassay kit Quantikine® Mouse Leptin Immunoassay kit Leptin

2 protocols using leptin immunoassay kit

1

Quantifying Serum Markers in Mice

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Serum insulin and leptin levels were quantified by a mouse insulin ELISA kit (Mercodia) and a leptin immunoassay kit (R&D Systems), respectively. Serum ALT and AST levels were quantified using the transaminase CII-test Wako kit (Wako).
Sato H., Taketomi Y., Ushida A., Isogai Y., Kojima T., Hirabayashi T., Miki Y., Yamamoto K., Nishito Y., Kobayashi T., Ikeda K., Taguchi R., Hara S., Ida S., Miyamoto Y., Watanabe M., Baba H., Miyata K., Oike Y., Gelb M.H, & Murakami M. (2014). The Adipocyte-Inducible Secreted Phospholipases PLA2G5 and PLA2G2E Play Distinct Roles in Obesity. Cell metabolism, 20(1), 119-132.
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2

Leptin Immunoassay Protocol for Adipose Tissue

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Leptin levels were determined using a leptin immunoassay kit (R&D Systems, Inc., Minneapolis, MN) according to the manufacturer’s protocol. The peri-renal AT was homogenized in lysate buffer (dilution buffer, included in the assay kit and protease inhibitor) at 500 μL per 100 mg of AT on ice and then centrifuged twice to remove lipids and debris. Rat serum was diluted 5 fold in dilution buffer, while AT was diluted 15-fold, and the supernatant containing adipocytes 4-fold.
Uchiyama T., Okajima F., Mogi C., Tobo A., Tomono S, & Sato K. (2017). Alamandine reduces leptin expression through the c-Src/p38 MAP kinase pathway in adipose tissue. PLoS ONE, 12(6), e0178769.
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