ATZ was acquired from Selleck Chemicals LLC, located in Houston, Texas, USA. Recombinant PD-L1 protein was obtained from R&D Systems in Lilly, France. The purity of PD-L1 protein was >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. Goat anti-human IgG was sourced from Sigma-Aldrich Chemical Co. in St. Louis, MO, USA. Additionally, goat anti-human horseradish peroxidase conjugate (HRP-IgG) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Chemicals Co. in St. Louis, Missouri, USA. Luminol was procured from ABCR GmbH in Karlsruhe, Germany, and 4-(imidazol-1-yl)phenol (IMP) was obtained from Sigma-Aldrich Chemicals Co. in St. Louis, MO, USA. Hydrogen peroxide was purchased from Merck in New Jersey, NJ, USA. White opaque flat-bottom high binding 96-well plates for chemiluminescence were obtained from Corning/Costar Inc. in Corning, NY, USA. Plasma samples were provided by King Khalid University Hospital in Riyadh, Kingdom of Saudi Arabia. These samples were stored frozen at −20 °C until they were utilized in the experiment. All other chemicals, reagents, and buffer components used throughout the study were of analytical grade.
4 imidazol 1 yl phenol imp
Manufactured by Merck Group
Sourced in Germany, China
4-(imidazol-1-yl)phenol (IMP) is a chemical compound used in various laboratory applications. Its core function is to serve as a reagent or intermediate in chemical synthesis and research. The product is available for purchase through Merck's catalog.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 4 imidazol 1 yl phenol imp
1
PD-L1 Protein Characterization and Quantification
2
Chemiluminescent Assay for Organophosphates
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Coumaphos, fenthion, chlorpyrifos, parathion, diazinon, fenchlorphos, fenitrothion, isocarbophos (ICP), ribavirin, amantadine, sulfamethoxydiazine, and oxytetracycline were purchased from the Chemical Metrology and Analytical Science Division of the National Institute of Metrology (Beijing, China), and 4-(imidazol-1-yl)phenol (IMP) and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich. Ethylene glycol dimethacrylate was purchased from Aladdin Industrial Corporation. Methacrylic acid (MAA), 2,2-azobis(isobutyronitrile), and polyvinyl alcohol were purchased from Tianjin Kemiou Chemical Reagent Co. Ltd. Luminol was purchased from Acros. An opaque 96-well microtiter plate was purchased from Jingan Biotechnology Co. Ltd. Other chemical reagents were purchased from Beijing Chemical Company, and all of them were of analytical grade or better.
The standard solutions of 7 OP were dissolved in methanol, and their working solutions (1 pg/mL to 1 mg/mL) were diluted with water. We prepared PBS (pH 7.2) by dissolving 0.2 g of KH 2 PO 4 , 0.2 g of KCl, 1.15 g of Na 2 HPO 4 , and 8.0 g of NaCl in 1,000 mL of water. The CL reagents (Luminol, H 2 O 2 , and IMP) were prepared with Tris-HCl (0.1 mol/L, pH 8.6), respectively.
The standard solutions of 7 OP were dissolved in methanol, and their working solutions (1 pg/mL to 1 mg/mL) were diluted with water. We prepared PBS (pH 7.2) by dissolving 0.2 g of KH 2 PO 4 , 0.2 g of KCl, 1.15 g of Na 2 HPO 4 , and 8.0 g of NaCl in 1,000 mL of water. The CL reagents (Luminol, H 2 O 2 , and IMP) were prepared with Tris-HCl (0.1 mol/L, pH 8.6), respectively.
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