SH-SY5Y cells, cultured on poly-L-lysine treated glass coverslip, were transfected at 60%–80% confluency with siRNAs for 48 h. Cells were fixed in 4% paraformaldehyde, permeabilized in 0.25% Triton-X, blocked in blocking buffer (3% BSA, 5% Normal Goat Serum (Thermo), 0.1% Tween) and incubated in primary antibody (TDP-43: 1:400; J2: 1:100). Slides were incubated with Rhodamine red anti-mouse and Cyanine 5 anti-rabbit secondary antibodies (Thermo Scientific; diluted 1:750) and were mounted in mounting media (Thermo). Cells were imaged with an Olympus FV1000 confocal microscope.
Rhodamine red anti mouse and cyanine 5 anti rabbit secondary antibodies
Manufactured by Thermo Fisher Scientific
Rhodamine red anti-mouse and Cyanine 5 anti-rabbit secondary antibodies are fluorescently-labeled antibodies used in immunoassays and other detection techniques. The rhodamine red label binds to mouse primary antibodies, and the Cyanine 5 label binds to rabbit primary antibodies, allowing for the visualization and detection of target proteins or cells.
Automatically generated - may contain errors
2 protocols using rhodamine red anti mouse and cyanine 5 anti rabbit secondary antibodies
1
Immunofluorescent Characterization of TDP-43
2
Immunofluorescent Characterization of TDP-43
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