Phosphorylated ampk

Manufactured by Abcam

Sourced in United States

Phosphorylated AMPK is a recombinant protein that represents the activated form of the 5' AMP-activated protein kinase (AMPK) enzyme. AMPK is a cellular energy sensor that becomes phosphorylated and activated in response to an increase in the AMP/ATP ratio, indicating a decrease in cellular energy levels.

Automatically generated - may contain errors

Lab products found in correlation

Srebp 1c, by Abcam (1 mentions) Phosphorylated lkb1, by Cell Signaling Technology (1 mentions) Goat anti rabbit secondary antibody, by Proteintech (1 mentions) Srebp2, by Abcam (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Lamin b, by Abcam (1 mentions) β actin, by Abcam (1 mentions)

2 protocols using phosphorylated ampk

Quantitative Analysis of Cholesterol Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from liver tissue or cultured cells using the RNeasy mini‐kit (QIAGEN, Maryland) and reverse‐transcribed into complementary DNA for quantitative real‐time PCR assay as described previously.⁽²²⁾To visualize the expressions of cholesterol‐related proteins, western blotting was used, and the primary antibodies against LDLR (diluted 1:1000; Abcam, Cambridge, United Kingdom), SREBP2 (diluted 1:2,000; Abcam), AMPK (diluted 1:1,000), phosphorylated AMPK (diluted 1:1,000; Abcam), ALR (diluted 1:1,000; Proteintech, Chicago, IL), phosphorylated LKB1 (diluted 1:500, Cell Signaling Technology, Beverly, MA), LKB1 (diluted 1:800, Abcam), SREBP1c (diluted 1:1,000; Abcam), and goat anti‐rabbit secondary antibody (diluted 1:5,000; Proteintech) were used.

Wang X., Dong L., Gai Q., Ai W., Wu Y., Xiao W., Zhang J, & An W. (2020). Lack of Augmenter of Liver Regeneration Disrupts Cholesterol Homeostasis of Liver in Mice by Inhibiting the AMPK Pathway. Hepatology Communications, 4(8), 1149-1167.

+ Open protocol

+ Expand

Quantification of Nrf2 and Related Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The process of protein quantification was performed using the Wes Simple Western System (ProteinSimple, San Jose, CA, USA) or the Western Blot technique as previous described [25 (link), 26 (link)]. The antibodies used in the study included nuclear factor erythroid 2-related factor 2 (Nrf2) (Abcam, Cambridge, MA, USA), β-actin (Abcam), Kelch-like ECH-associated protein 1 (Keap1) (Abcam), AMP-activated protein kinase (AMPK) (Abcam), phosphorylated AMPK (Abcam), lamin B (Abcam), and p62 (Abcam). The mouse β-actin antibody was used as a loading control for total protein, while nuclear Nrf2 protein expression was normalized to lamin B. All protein concentrations were determined using a standard BCA protein assay. Results of Wes Simple Western System were obtained using the “gel view” function of the Protein Simple software (ProteinSimple). Western blot data were quantified using the ImageJ software.

Tang W., Long J., Li T., Yang L., Li J., He L., Li S., Kuang S., Feng Y., Chen H., Li F., Du Z, & Yin Y. (2020). The Associated Regulatory Mechanisms of Zinc Lactate in Redox Balance and Mitochondrial Function of Intestinal Porcine Epithelial Cells. Oxidative Medicine and Cellular Longevity, 2020, 8815383.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!