Chiloscyllium plagiosum and Mustelus griseus were derived from Xiamen Tekwon Genetic Technologies Ltd. and frozen at −20℃ for preservation. Papain (2,000 U/mg), Hematoxylin Staining Solution, and Eosin Staining Solution were purchased from Sangon Biotech Co., Ltd., Shanghai, China. Optimal cutting temperature (O.C.T) was obtained from Sakura Finetek, Inc., Torrance, USA. Pentobarbital sodium was from Sigma‐Aldrich, USA. All other reagents utilized in this research were of analytical grade.
Eosin staining solution
Manufactured by Sangon
Sourced in China
Eosin Staining Solution is a laboratory reagent used for staining biological samples in histology and cytology applications. It is a water-soluble dye that binds to basic structures in cells, such as proteins, and imparts a pink or red color. The solution is commonly used in combination with other stains, such as hematoxylin, to provide contrast and aid in the visualization of cellular structures under a microscope.
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2 protocols using eosin staining solution
1
Cartilaginous Fish Tissue Preservation
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Chiloscyllium plagiosum and Mustelus griseus were derived from Xiamen Tekwon Genetic Technologies Ltd. and frozen at −20℃ for preservation. Papain (2,000 U/mg), Hematoxylin Staining Solution, and Eosin Staining Solution were purchased from Sangon Biotech Co., Ltd., Shanghai, China. Optimal cutting temperature (O.C.T) was obtained from Sakura Finetek, Inc., Torrance, USA. Pentobarbital sodium was from Sigma‐Aldrich, USA. All other reagents utilized in this research were of analytical grade.
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Chiloscyllium plagiosum and Mustelus griseus were derived from Xiamen Tekwon Genetic Technologies Ltd. and frozen at −20℃ for preservation. Papain (2,000 U/mg), Hematoxylin Staining Solution, and Eosin Staining Solution were purchased from Sangon Biotech Co., Ltd., Shanghai, China. Optimal cutting temperature (O.C.T) was obtained from Sakura Finetek, Inc., Torrance, USA. Pentobarbital sodium was from Sigma‐Aldrich, USA. All other reagents utilized in this research were of analytical grade.
2
Histological Processing of Ovarian Tissues
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Ovarian tissues underwent fixation in 4% paraformaldehyde at room temperature for 48 h, and were subsequently dehydrated in a series of ethanol concentrations (70%, 80%, 90%, and 100%). Following dehydration, the tissues were immersed in a solution of xylene and ethanol (1∶1) and then in 100% xylene. The ovarian tissues were embedded in paraffin and sectioned into 5 μm thick slides. Hematoxylin and eosin (H&E) staining was carried out using hematoxylin staining solution (Cat. #G1004, Servicebio, Wuhan, China) and eosin staining solution (Cat. #E607321, Sangon Biotech, Shanghai, China).
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