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Criterion precast page gels

Manufactured by Bio-Rad
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Criterion precast PAGE gels are polyacrylamide gel electrophoresis (PAGE) products manufactured by Bio-Rad. They are used for the separation and analysis of proteins based on their molecular weight.

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Lab products found in correlation

Gapdh, by Thermo Fisher Scientific (2 mentions) Pthr308 akt, by Cell Signaling Technology (2 mentions) P akt ser473, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Criterion TGX precast SDS-PAGE gels Criterion precast gels Criterion gels Precast gels PAGE gels

2 protocols using criterion precast page gels

1

Western Blotting of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For western blotting, liver lysates were collected in lysis buffer (150 mM NaCl, 20 mM Tris (pH = 7.4), 1 mM EDTA, 0.2% NP-40, 10% glycerol) with protease inhibitors using a Tissuelyser. Lysates were normalized to protein concentration, denatured, and run on Criterion precast PAGE gels (BioRad). The antibodies used in this study were ALT2 (MilliporeSigmaHPA051514), ALT1 (GPT Abcam ab202083), Complex III (Oxphos Cocktail; Abcam ab110413), GAPDH (Invitrogen AM4300), AKT (Cell Signaling Technology 2920), pThr308 AKT (Cell Signaling Technology 2920), pSer473 AKT (Cell Signaling Technology 2920), or tubulin (MilliporeSigmaMonoclonal Anti-α- Tubulin Clone B-5-1-2, T51668).
Martino M.R., Gutiérrez-Aguilar M., Yiew N.K., Lutkewitte A.J., Singer J.M., McCommis K.S., Ferguson D., Liss K.H., Yoshino J., Renkemeyer M.K., Smith G.I., Cho K., Fletcher J.A., Klein S., Patti G.J., Burgess S.C, & Finck B.N. (2022). Silencing alanine transaminase 2 in diabetic liver attenuates hyperglycemia by reducing gluconeogenesis from amino acids. Cell reports, 39(4), 110733.
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2

Western Blotting of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
For western blotting, liver lysates were collected in lysis buffer (150 mM NaCl, 20 mM Tris (pH = 7.4), 1 mM EDTA, 0.2% NP-40, 10% glycerol) with protease inhibitors using a Tissuelyser. Lysates were normalized to protein concentration, denatured, and run on Criterion precast PAGE gels (BioRad). The antibodies used in this study were ALT2 (MilliporeSigmaHPA051514), ALT1 (GPT Abcam ab202083), Complex III (Oxphos Cocktail; Abcam ab110413), GAPDH (Invitrogen AM4300), AKT (Cell Signaling Technology 2920), pThr308 AKT (Cell Signaling Technology 2920), pSer473 AKT (Cell Signaling Technology 2920), or tubulin (MilliporeSigmaMonoclonal Anti-α- Tubulin Clone B-5-1-2, T51668).
Martino M.R., Gutiérrez-Aguilar M., Yiew N.K., Lutkewitte A.J., Singer J.M., McCommis K.S., Ferguson D., Liss K.H., Yoshino J., Renkemeyer M.K., Smith G.I., Cho K., Fletcher J.A., Klein S., Patti G.J., Burgess S.C, & Finck B.N. (2022). Silencing alanine transaminase 2 in diabetic liver attenuates hyperglycemia by reducing gluconeogenesis from amino acids. Cell reports, 39(4), 110733.
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