Male 12-week-old Sprague-Dawley (SD; Charles River, Sulzfeld, Germany), Zucker Diabetic Fatty (ZDF), and ZDF lean rats (Charles River, Kingston, NY) were housed in a room at 22 ± 2°C under 12-h light/dark cycles. Sprague-Dawley rats were fed a standard laboratory rat diet and ZDF rats a diet of Purina 5008 as recommended by the supplier and water ad libitum. The rats were acclimatized for at least 1 week before the experiments. All animals received humane care in compliance with the Principles of Laboratory Animal Care formulated by the National Society for Medical Research and the Guide for the Care and Use of Laboratory Animals prepared by the Institute of Laboratory Animal Resources and published by the National Institutes of Health (NIH Publication no. 86-23, revised 1996). This investigation was reviewed and approved by the appropriate institutional review committee.
Zucker diabetic fatty
Manufactured by Charles River Laboratories
Sourced in United States
The Zucker diabetic fatty (ZDF) rat is a genetic model used in research. It exhibits characteristics of type 2 diabetes, including obesity, hyperglycemia, and insulin resistance.
Automatically generated - may contain errors
Lab products found in correlation
Pdgf bb, by Merck Group (2 mentions)
Male zucker lean rats, by Charles River Laboratories (2 mentions)
Zucker fatty rats, by Charles River Laboratories (2 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (2 mentions)
Zdf lean rats, by Charles River Laboratories (1 mentions)
Sprague dawley, by Charles River Laboratories (1 mentions)
Crf 1, by Oriental Yeast (1 mentions)
5 protocols using zucker diabetic fatty
1
Dietary Influences on Sprague-Dawley and Zucker Rats
2
Zucker Rat Diabetes Study Protocol
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All animal experimental protocols were approved by the regional Animal Care and Use Committee and performed according to the Guide for the Care and Use of Laboratory Animals (120/13) [15 ]. For this study, 12 rats of 13 weeks of age (ZL: Zucker Lean control (n = 6), ZDF: Zucker diabetic fatty (n = 6), Charles River) were included (mean body weight: 336 ± 61 g).
3
Zucker Rat Animal Study Protocol
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All protocols for animal use and euthanasia were reviewed and approved by the Animal Care Committee of the Joslin Diabetes Center. The experiments were in accordance with National Institutes of Health (NIH) guidelines following the standards established by the Animal Welfare Acts and by the documents entitled ‘Principles for Use of Animals’ and ‘Guide for the Care and Use of Laboratory Animals’. Male Zucker lean (ZL) rats, Zucker fatty (ZF) rats, Zucker diabetic fatty (ZDF) and their control (LEAN +/?) were from Charles River (Wilmington, MA, USA). C57BL/6 mice were from Jackson Laboratory (Bar Harbor, ME, USA). PDGF-BB was purchased from Sigma (St Louis, MO, USA).
4
Animal Models of Kidney Injury and Diabetes
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Male Sprague Dawley (SD), Zucker lean (ZL) and Zucker diabetic fatty (ZDF) rats were purchased from Charles River Laboratories, Inc. Prior to the experimental procedures, animals were acclimatized for at least 5 days and housed under a 12-h light/dark cycle with ad libitum access to water and standard chow, CRF-1 (Oriental Yeast Co., Ltd.). For the puromycin aminonucleoside (PAN)-induced glomerular nephritis model, 73 6-week-old male SD rats received a tail vein injection of 100 mg/kg/5 ml PAN (Sigma-Aldrich; Merck KGaA) in saline buffer. In addition, 24 saline buffer-injected rats were used as the control. Prednisolone was administered as a single oral dose of 10 mg/kg/10 ml prednisolone (Shionogi & Co., Ltd.) 1 h prior to PAN injection using distilled water as the vehicle control. These animals were kept for 1 week and then were euthanized. For the type 2 diabetes model, 12-week-old male ZL (n=6) and ZDF (n=12) rats were used at the beginning of the experiment. These animals were kept for 20 weeks and then were euthanized. All animals were observed at least once daily for monitoring of health. No unforeseen deaths of animals occurred in these studies.
5
Zucker Rat Diabetes Animal Protocol
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