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Legendplex cytokine analysis kit

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The LEGENDplex Cytokine Analysis kit is a multiplex bead-based assay that enables the simultaneous quantification of multiple cytokines, chemokines, and other soluble analytes in a single sample. The kit utilizes fluorescent-coded beads and a flow cytometry-based platform to provide a powerful and efficient tool for cytokine profiling.

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Lab products found in correlation

Lsrii fortessa, by BD (1 mentions) Facs canto 1 cytometer, by BD (1 mentions)

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LEGENDplex (292 citations) LEGENDplex™ kit (64 citations)

2 protocols using legendplex cytokine analysis kit

1

Cytokine Profiling in Mouse Blood Serum

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Experimental mice were euthanized through CO2 asphyxiation and cervical dislocation and whole blood was collected via cardiac puncture. Blood was allowed to coagulate for 30 min at room temperature and spun at 1000 g for 10 min to separate the serum. Serum was then transferred to a new 1.5 mL eppendorf tube. Cytokine analysis was performed using the LEGENDplex Cytokine Analysis kit (BioLegend) according to manufacturer recommendations and analyzed on the Sony SH800 or BD LSRII Fortessa. Flow cytometry results were converted to pg/mL secretion using BioLegend LEGENDplex software.
Fromm G., de Silva S., Johannes K., Patel A., Hornblower J.C, & Schreiber T.H. (2018). Agonist redirected checkpoint, PD1-Fc-OX40L, for cancer immunotherapy. Journal for Immunotherapy of Cancer, 6, 149.
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2

Cytokine Profiling of Lung Supernatants

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Supernatants obtained from fresh lung suspensions (total volume 3 mL) were aliquoted and frozen at −80°C. The aliquots were thawed (only once) and the cytokines present in a 25 μL sample were analyzed (CXCL1, IL-18, IL-23, IL-12p70, IL-6, TNF-α, IL-1β, and IL-12p40) with the LEGENDplex Cytokine Analysis kit (BioLegend), according to the manufacturer’s recommendations, and using a FACS Canto I cytometer (BD Biosciences) and the BioLegend LEGENDplex software.
Sánchez-Tarjuelo R., Cortegano I., Manosalva J., Rodríguez M., Ruíz C., Alía M., Prado M.C., Cano E.M., Ferrándiz M.J., de la Campa A.G., Gaspar M.L, & de Andrés B. (2020). The TLR4-MyD88 Signaling Axis Regulates Lung Monocyte Differentiation Pathways in Response to Streptococcus pneumoniae. Frontiers in Immunology, 11, 2120.
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