Normal and OA cartilage were cultured in a complete medium for 24 h, then the complete medium was replaced by a serum-free medium. After 2 h, the conditioned medium was harvested, centrifuged to remove particulate matter, and stored at −20 °C until analyzed. Quantitation of TNF-α secreted into the medium was determined using a quantitative sandwich ELISA for human TNF-α according to the manufacturer's instructions (R&D Systems, Minneapolis, MN).
Quantitative sandwich elisa for human tnf α
Manufactured by R&D Systems
The Quantitative sandwich ELISA for human TNF-α is a laboratory equipment product that measures the concentration of human Tumor Necrosis Factor-alpha (TNF-α) in biological samples. It utilizes the sandwich ELISA technique to quantify the target analyte.
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Lab products found in correlation
2 protocols using quantitative sandwich elisa for human tnf α
1
Quantifying TNF-α Secretion in Cartilage
2
Quantifying TNF-α Secretion in Cartilage
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Normal and OA cartilage were cultured in a complete medium for 24 h, then the complete medium was replaced by a serum-free medium. After 2 h, the conditioned medium was harvested, centrifuged to remove particulate matter, and stored at −20 °C until analyzed. Quantitation of TNF-α secreted into the medium was determined using a quantitative sandwich ELISA for human TNF-α according to the manufacturer's instructions (R&D Systems, Minneapolis, MN).
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