Dulbecco s modified eagle s minimal essential medium dmem

Manufactured by Merck Group

Sourced in France, United States

Dulbecco's Modified Eagle's minimal essential medium (DMEM) is a cell culture medium formulation that provides a balanced salt solution and nutrients to support the growth and maintenance of various cell types in vitro. It is a widely used medium in the field of cell biology and biopharmaceutical research.

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Lab products found in correlation

25 cm2 tissue culture flask, by Thermo Fisher Scientific (1 mentions) Penicillin g streptomycin, by Merck Group (1 mentions) Maltose, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cellobiose, by Merck Group (1 mentions) Cysteine, by Merck Group (1 mentions)

Close spelling variants of this product

Dulbecco’s modified Eagle’s minimal essential medium Dulbecco’s minimal essential medium (DMEM, Dulbecco’s modified essential medium (DMEM, Dulbecco’s minimal essential medium Dulbecco’s modified essential medium Dulbecco minimal essential medium (DMEM) Dulbecco‐modified essential medium (DMEM) Minimal essential medium Dulbecco’s modified Minimal DMEM

2 protocols using dulbecco s modified eagle s minimal essential medium dmem

Growth of Faecalibacterium prausnitzii Strains

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The reference strains A2-165 (DSM 17677; Duncan et al., 2002 (link)), L2/6 (Barcenilla et al., 2000 (link)) and M21/2 (Louis et al., 2004 (link)) and the F. prausnitzii isolated strains (Table 1) were grown at 37°C in YBHI medium supplemented with cellobiose (1 mg/ml; Sigma), maltose (1 mg/ml; Sigma), and cysteine (0.5 mg/ml; Sigma) in an anaerobic chamber filled with N₂ = 90%, CO₂ = 5% and H₂ = 5%.
HT-29 (ATCC HTB-38) (LGC-Standars) cell line was grown in Dulbecco's Modified Eagle's minimal essential medium (DMEM) (Sigma-Aldrich) supplemented with 10% (w/v) heat-inactivated fetal bovine serum (FBS) (GibcoBRL, Eragny, France) and with penicillin G/ streptomycin (5,000 IU/mL, 5,000 μg/mL) (Sigma-Aldrich). Cultures were incubated in 25 cm² tissue culture flasks (Nunc, Roskilde, Denmark) at 37°C in a 5% (v/v) CO₂ atmosphere until confluence.

Martín R., Miquel S., Benevides L., Bridonneau C., Robert V., Hudault S., Chain F., Berteau O., Azevedo V., Chatel J.M., Sokol H., Bermúdez-Humarán L.G., Thomas M, & Langella P. (2017). Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic. Frontiers in Microbiology, 8, 1226.

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Photosensitizer Synthesis and Preparation

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The photosensitizers used in this study were RF (Sigma-Aldrich, St. Louis, USA) and RFTA, which was obtained as previously described [19] with modification of the synthetic procedure [31] . Briefly, 0.5 g of RF was mixed with 100 mL of (CH 3 CO) 2 O:C 2 H 4 O 2 (1:1), and then 1 mL of HClO 4 was added and the mixture was refluxed for 3 h at 40 °C. The reaction was cooled to 0 °C, after 200 mL of cold deionizer water was added and extracted three times with 25 mL CHCl 3 . The organic phase was washed with 25 mL of cold water followed by 25 mL of a cold saturated NaCl solution. The solvent was evaporated at 50 °C and the product recrystallized four times in CH 3 OH:H 2 O (17:3). Finally, RFTA was diluted in 1% (v/v) acetone-high glucose Dulbecco's modified Eagle's minimal essential medium (DMEM; Sigma-Aldrich, St. Louis, USA).

Juarez A.V., Sosa Ldel V., De Paul A.L., Costa A.P., Farina M., Leal R.B., Torres A.I, & Pons P. (2015). Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy. Journal of photochemistry and photobiology. B, Biology, 153.

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