The mono and oligosaccharide profiles from the supernatants of C. vulgaris after control and Mix treatments were analysed by High Performance Liquid Chromatography (HPLC) on an Agilent system (Agilent 1200 Series, Agilent Technologies Inc., Palo Alto, CA), equipped with an electrochemical detector (Coulochem III, ESA Dionex Thermo Fisher Scientific Inc, USA). The HPLC analysis was performed using a Dionex CarboPac PA10 column (4 × 250 mm, Thermo Fisher Scientific Inc, USA) fitted to a CarboPac PA10 guard column (4 × 50 mm), following the procedure described by Thermo Fisher Scientific46 with slight modifications. The separation of mono and oligosaccharides was achieved using a mobile phase with a flow rate of 1 mL/min for 60 min at 25 °C, as follows: isocratic elution with 18 mM NaOH (eluent A) during 18 min, gradient with 100–0 mM NaOH (eluent B) and 0–75 mM sodium acetate in 100 mM NaOH (eluent C) from 18–40 min, and re-equilibration to 18 mM NaOH during 20 min. The quantification of total oligosaccharides was based on a standard curve, using a range of concentrations from 0.025 mM to 0.2 mM of glucose. The results were expressed as equivalent moles of glucose released per gram of microalgae.
Carbopac pa10 guard column
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CarboPac PA10 guard column is a component used in high-performance anion-exchange chromatography (HPAE) systems. It is designed to protect the analytical column from contamination and fouling, thereby extending the column's lifetime and performance. The guard column is typically placed in front of the analytical column to trap and remove unwanted sample components before they reach the main separation column.
Automatically generated - may contain errors
Lab products found in correlation
Carbopac pa10 column, by Thermo Fisher Scientific (2 mentions)
Dionex carbopac pa10 column, by Thermo Fisher Scientific (1 mentions)
Agilent 1200 series, by Agilent Technologies (1 mentions)
Dionex biolc, by Thermo Fisher Scientific (1 mentions)
Carbopac pa200 column, by Thermo Fisher Scientific (1 mentions)
Dionex ics 5000, by Thermo Fisher Scientific (1 mentions)
Carbopac pa200 guard column, by Thermo Fisher Scientific (1 mentions)
Carbopac pa10, by Thermo Fisher Scientific (1 mentions)
4 protocols using carbopac pa10 guard column
1
HPLC Analysis of Microalgae Oligosaccharides
2
HPAEC-PAD Analysis of Oxidized Cellulose
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glucose and cellobiose and higher oligosaccharides were analyzed with high performance anion exchange chromatography coupled to pulsed-amperometric detection (HPAEC-PAD) (Dionex BioLC, Thermo Fisher Scientific, Waltham, MA, USA)38 .
The production of oxidized cellulose-oligosaccharides was monitored with the same HPAEC-PAD system equipped with a CarboPac® PA10 column (4 × 250 mm) and a CarboPac PA10 guard column (4 × 50 mm) at 30 °C. Elution of uncharged saccharides was performed at 0.7 ml min−1 using 50 mM sodium hydroxide and 20 mM sodium acetate in the mobile phase for 16 min followed by a sodium acetate gradient. Aldonic acids were eluted by a linear gradient from 40 mM up to 400 mM sodium acetate at a flow of 0.7 ml min−1 over 20 min. Afterwards, the column was re-equilibrated for nine minutes with 50 mM sodium hydroxide and 20 mM sodium acetate.
d -Glucose, d -cellobiose and d -gluconic acid were used as authentic standards. Identification or quantification of oxidized products was not pursued.
The production of oxidized cellulose-oligosaccharides was monitored with the same HPAEC-PAD system equipped with a CarboPac® PA10 column (4 × 250 mm) and a CarboPac PA10 guard column (4 × 50 mm) at 30 °C. Elution of uncharged saccharides was performed at 0.7 ml min−1 using 50 mM sodium hydroxide and 20 mM sodium acetate in the mobile phase for 16 min followed by a sodium acetate gradient. Aldonic acids were eluted by a linear gradient from 40 mM up to 400 mM sodium acetate at a flow of 0.7 ml min−1 over 20 min. Afterwards, the column was re-equilibrated for nine minutes with 50 mM sodium hydroxide and 20 mM sodium acetate.
3
Oligosaccharide and Lactose Quantification by HPAEC-PAD
4
Intestinal Permeability Evaluation Using Sugar Probes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the evaluation of intestinal permeability, a test solution was prepared containing 40 g sucrose (Su), 10 g lactulose (La), and 5 g mannitol (Ma) dissolved in 100 ml of water.
The participants drank the test solution in the morning after an overnight fast and all urine samples were collected for the subsequent five hours. Urine samples were stored at −80°C until analysis. The detection and measurement of the three sugar probes, Su, La, and Ma, in urine were performed by chromatographic analysis as described previously by our group [23 (link)]. Briefly, high-performance anion exchange chromatography coupled with pulsed amperometric detection was performed on a Dionex Model ICS-5000 with a gold working electrode and a 25 μl peek sample loop (Dionex Corp., Sunnyvale, California, USA).
The carbohydrate separation was performed using a CarboPac PA-10 pellicular anion-exchange resin connected to a CarboPac PA-10 guard column (Thermofisher Scientific, Waltham, Massachusetts, USA) at 30°C. The samples were eluted with 50 mmol/l NaOH at a flow rate of 1 ml/min. The percentages of ingested Su (%Su) together with those of La (%La) and Ma (%Ma) in urine were evaluated, and the La/Ma ratio was calculated for each sample. Based on data from controls in our laboratory, La/Ma ≥ 0.035 is indicative of increased intestinal permeability [23 (link)].
The participants drank the test solution in the morning after an overnight fast and all urine samples were collected for the subsequent five hours. Urine samples were stored at −80°C until analysis. The detection and measurement of the three sugar probes, Su, La, and Ma, in urine were performed by chromatographic analysis as described previously by our group [23 (link)]. Briefly, high-performance anion exchange chromatography coupled with pulsed amperometric detection was performed on a Dionex Model ICS-5000 with a gold working electrode and a 25 μl peek sample loop (Dionex Corp., Sunnyvale, California, USA).
The carbohydrate separation was performed using a CarboPac PA-10 pellicular anion-exchange resin connected to a CarboPac PA-10 guard column (Thermofisher Scientific, Waltham, Massachusetts, USA) at 30°C. The samples were eluted with 50 mmol/l NaOH at a flow rate of 1 ml/min. The percentages of ingested Su (%Su) together with those of La (%La) and Ma (%Ma) in urine were evaluated, and the La/Ma ratio was calculated for each sample. Based on data from controls in our laboratory, La/Ma ≥ 0.035 is indicative of increased intestinal permeability [23 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!