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2 protocols using β terpinene

1

Phytochemical Extraction and Characterization

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Phytochemicals such as 1-cyclohexyl-2-pyrrolidone, camphene, cinnamaldehyde, citral, geraniol, L-carvone, L-limonene, L-menthol, β-myrcene, ocimene, α-terpinene, and β-terpinene (Figure 1) and other essential chemicals were purchased from Sigma-Aldrich Chemicals Pvt. Ltd., India.
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2

Identification of Volatile Organic Compounds

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The synthetic standards including hexanal, α-thujene, α-pinene, camphene, o-cymene, sabinene, β-pinene, myrcene, α-terpinene, β-terpinene, terpinolene, decanal, tridecane, pentadecane, cedrol, hexadecane and the solvent dichloromethane were purchased from Sigma Aldrich (USA) whilst δ-2-carene, α-phellandrene, δ-3-carene, p-cymene, limonene, β- phellandrene, β-ocimene [mixture of (E) and (Z)], dihydrotagetone, δ-elemene, β-elemene, α-cedrene, (E)-caryophyllene, α-humulene, (E, E)-α-farnesene were purchased from Merck (France) and were used to confirm the identified compounds. The chemical purity of the synthetic standards except for sabinene (75%) and α-phellandrene (85%) ranged between 90–99%.
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