Tissue samples were fixed in formalin for 1–3 days, and then transferred to 70% alcohol. Samples were embedded in wax and cut into 5-µm sections. Sections were stained with hematoxylin-eosin (H-E). For fibrosis, sections were stained with Masson Trichrome reagent, and imaged and analyzed using a Vectra Intelligent Multispectral Slide Analysis System (Perkin-Elmer, Waltham, MA). For calcification, aorta sections were stained by the von Kossa method and counterstained with nuclear fast red (Wu-Wong et al., 2007 (link)).
Vectra intelligent multispectral slide analysis system
Manufactured by PerkinElmer
The Vectra Intelligent Multispectral Slide Analysis System is a lab equipment product that enables multispectral imaging and analysis of tissue samples. It provides a comprehensive platform for automated slide scanning, high-resolution imaging, and advanced data analysis.
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Lab products found in correlation
2 protocols using vectra intelligent multispectral slide analysis system
1
Histopathological Analysis of Tissue Samples
2
Histological Analysis of Tissue Samples
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Tissue samples were fixed in formalin for 1–3 days, and then transferred to 70% alcohol. Samples were embedded in wax and cut into 5-μm sections. Sections were stained with hematoxylin-eosin (H-E). For fibrosis, sections were stained with Masson Trichrome reagent, and imaged and analyzed using a Vectra Intelligent Multispectral Slide Analysis System (Perkin-Elmer, Waltham, MA). For calcification, aorta sections were stained by the von Kossa method and counterstained with nuclear fast red (Wu-Wong et al. 2007 (link)).
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