Axiocam 503 ccd camera

Manufactured by Zeiss

The Axiocam 503 is a high-performance CCD camera developed by Zeiss. It features a 5-megapixel sensor and is designed for microscopy applications. The camera provides a fast data transfer rate and supports a range of resolutions and frame rates to meet the needs of various imaging tasks.

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Lab products found in correlation

Axio observer a1 inverted, by Zeiss (1 mentions) Micro slide, by Ibidi (1 mentions) Dfc3000g camera, by Leica (1 mentions) Axio observer a1 microscope, by Zeiss (1 mentions) Dmi3000b microscope, by Leica (1 mentions) Cellview cell culture dish, by Greiner (1 mentions)

Close spelling variants of this product

Axiocam 503 camera AxioCam CCD camera Axiocam 503 AxioCam camera CCD camera AxioCam

2 protocols using axiocam 503 ccd camera

3D Tracking of Microswimmer Cocultures

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By keeping C. reinhardtii (puller-type
microswimmer) density constant, we varied E. coli (pusher-type microswimmer) density in a high throughput manner using
an 8-well microchamber (Micro-Slide, ibidi, Germany) and recorded
time-lapse videos for 9 h. C. reinhardtii can be effectively tracked in 3D because of their intrinsic autofluorescence
properties, with live imaging to extract various quantitative information.
The 3D trajectories were generated by using a Nikon NIS 3D object
tracking module. The motions of the mixed microswimmer populations
were examined inside a custom-made microscope chamber slide harboring
8 wells (bottom thickness = 150 μm) for high-throughput analysis.
The microscopy investigation was performed with a Zeiss Axio Observer
A1 inverted microscope with an Axiocam 503 CCD camera and a 40×
(numerical aperture = 0.6) objective lens. The microalgae and bacteria
cocultures were illuminated by using a red filter with an emission
peak at 655 nm, and a bandwidth of 15 nm (655/15 BrightLine HC, AHF
Analysentechnik, Tübingen, Germany), to prevent phototactic
biased motion of the microalgae and thus to avoid false-positive results.^{15 (link)}

Singh A.V., Kishore V., Santomauro G., Yasa O., Bill J, & Sitti M. (2020). Mechanical Coupling of Puller and Pusher Active Microswimmers Influences Motility. Langmuir, 36(19), 5435-5443.

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Magnetically Guided Bacterial Microswimmers

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Microtubes were added to bacteria in motility media and allowed to incubate at 37°C and 150 rpm for 30 min. The solutions containing the biohybrid swimmers were imaged in petri dishes with glass coverslip bottoms (Cellview Cell Culture Dish, Greiner Bio-One) at ambient temperature. Videos and images were acquired using bright field microscopy with an inverted Leica (Wetzlar, Germany) DMI3000B microscope, Leica DFC3000G camera, and Leica Application Suite v.4.5.0 software. For magnetically guided microswimmers, motion control experiments were conducted using an electromagnetic coil system. The system consisted of four orthogonally oriented iron-core electromagnets. The coils were built onto a custom-made microscope stage to surround a 35 mm Petri dish and was placed onto an inverted Zeiss (Carl Zeiss, Inc., Oberkochen, Germany) Axio Observer A1 microscope with a 20x objective (Figure 4 and Figure S1). The input current for the coils was controlled by motor drivers and an Arduino microcontroller board, and the ~8 mT magnetic field strength was calibrated using a Lake Shore Cryotronics (Darmstatdt, Germany) Model 460-3-Channel Gaussmeter.
Microswimmers were recorded with a Zeiss Axiocam 503 CCD camera.

Stanton M.M., Park B.W., Miguel-López A., Ma X., Sitti M, & Sánchez S. (2017). Biohybrid Microtube Swimmers Driven by Single Captured Bacteria. Small (Weinheim an der Bergstrasse, Germany), 13(19).

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