Transwell room

According to the manufacturer's instructions, we inoculated processed YY8103 and Hep3B cells into 200 μl serum-free DMEM medium in the upper chamber. The transwell room (Corning, USA) accepted the paving process, used Matrigel mix (BD Biosciences, USA) for the invasion test process, and did not use the Matrigel mix for the migration test. DMEM medium and 10% FBS are introduced into the bottom chamber to become HCC cytochemical attractants. When the 48-hour incubation process is completed, the upper chamber is fixed and then stained with crystal violet (Kagan, China) for 15 minutes. For the visualization process, the cell line received the photo and counting process in three fields.

Cells (AN3-CA) used in the experiment were provided by Wuhan University Cell Bank; FBS and dmem were purchased from Gibco; 6-well plates were purchased from Corning; the Transwell Room was purchased from Corning; the CCK8 cytotoxicity kits were purchased from Toyobo Life Science, and capsaicin was purchased from Shanghai universal Biotech Co.,Ltd.