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4600 spectrofluorimeter

Manufactured by Hitachi

The 4600 spectrofluorimeter is a laboratory instrument manufactured by Hitachi. It is designed to measure the fluorescence properties of samples. The core function of the 4600 spectrofluorimeter is to excite samples with light and detect the emitted fluorescence, providing quantitative data on the sample's fluorescent characteristics.

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2 protocols using 4600 spectrofluorimeter

1

Spectroscopic Analysis of Metal Ion Complexes

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The reagents used in the experiment process were commercially available and used directly. The metal ions salts employed are NaCl, KCl, CaCl2·2H2O, MgCl2·6H2O, CdCl2, HgCl2, FeCl3·6H2O, CrCl3·6H2O, Zn(NO3)2·6H2O, AgNO3, CoCl2·6H2O, MnCl2·4H2O, CuCl2·2H2O, NiCl2·6H2O, and PbCl2. The anions salts employed are NaClO, Na2SO4, NaNO3, Na2CO3, NaCl, NaAc, NaClO4, NaBr, KI, NaSCN, and Na2HPO4, respectively.
UV-vis absorption spectra and fluorescence emission spectra were recorded on Hitachi U-2910 spectrophotometer and Hitachi 4600 spectrofluorimeter at 25 °C with 1 cm quartz cell, respectively. Mass spectra were collected on a Thermo TSQ Quantum Access Agillent 1100 system. Nuclear magnetic resonance spectra were performed with a Bruker AV 400 instrument (400 MHz), and chemical shifts were given in ppm by using tetramethylsilane (TMS) as internal standards.
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2

Metal Ion-Induced Fluorescence Assay

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All reagents and solvents are of analytical grade and used without further purification. The metal ions employed are NaCl, KCl, CaCl2·2H2O, MgCl2·6H2O, Zn(NO3)2·6H2O, PbCl2, CdCl2, CrCl3·6H2O, CoCl2·6H2O, NiCl2·6H2O, HgCl2, CuCl2·2H2O, FeCl3·6H2O and AgNO3, respectively.
Fluorescence emission spectra were conducted on a Hitachi 4600 spectrofluorimeter. UV-Vis spectra were obtained on a Hitachi U-2910 spectrophotometer. Nuclear magnetic resonance (NMR) spectra were measured with a Bruker AV 400 instrument and chemical shifts are given in ppm from tetramethylsilane (TMS). Mass (MS) spectra were recorded on a Thermo TSQ Quantum Access Agillent 1100 system.
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