Bioamp powerlab 8 35

The anesthesia used to perform the ECGs was ketamine (64 μg/g body weight)/xylazine (10 μg/g) in PBS (i.p.). After 15 min, electrocardiograms were obtained from mice using BioAmp PowerLab 8/35 (AD Instruments, Dunedin, New Zealand) for the data acquisition. The negative electrode and positive electrode were placed in the upper right and lower left limbs, respectively. The neutral pole was placed in the lower right limb. We analyzed a section of the ECG for 1 min and we used the ECG module of the LabChart 8.0 software and the preset mouse settings. The number of analyzed mice is detailed in Tables 1 and 3.

Electrocardiograms were obtained from anesthetized mice using the data acquisition system BioAmp PowerLab 8/35 (ADInstruments). Negative and positive electrodes were placed in the upper right and lower left limbs, respectively, and the neutral pole was placed in the lower right limb. We analyzed a section of the ECG (about 1 min) using the ECG module of the LabChart 8.0 software and the preset mouse settings.
MRI experiments were carried on a Bruker BioSpec MRI system (Bruker BioSpec, Bruker BioSpin, Ettlingen, Germany) equipped with 400 mT m-1 field gradients and a 40 mm quadrature bird-cage resonator. Cine short-axis (SAX) and long-axis (LAX) were acquired using a retrospective self-gated FLASH sequences (IntraGate, Bruker BioSpin MRI, Ettlingen, Germany). The acquisition parameters were as follows: α = 20°, TE = 2.88 ms, TR = 8.5 ms, FOV = 11 × 22 mm, matrix size = 192 × 384, movie frames = 16, number of slices = 1, and slice thickness = 0.8.