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Anti nitrotyrosine antibody

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The anti-nitrotyrosine antibody is a laboratory reagent used to detect and quantify the presence of nitrotyrosine, a protein modification that can indicate oxidative stress or nitric oxide signaling in biological samples. It is a specific and sensitive tool for researchers studying these cellular processes.

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Lab products found in correlation

Lsab kit peroxidase, by Agilent Technologies (2 mentions) Cleaved caspase 3 asp175 antibody, by Cell Signaling Technology (1 mentions) Dako lsab peroxidase kit, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Anti-nitrotyrosine (2 citations) Anti-nitrotyrosine primary antibody (A-21285, (2 citations)

3 protocols using anti nitrotyrosine antibody

1

Histological Analysis of Liver Tissue

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Formalin-fixed liver tissues were embedded in paraffin and 5 µm thick sections were cut. Hematoxylin and eosin (H&E) staining was performed for evaluation of tissue necrosis (Gujral et al., 2002 (link)). Nitrotyrosine staining was performed for assessment of nitrotyrosine (NT) protein adducts, as previously described (Knight et al., 2002 (link)), using the Dako LSAB peroxidase kit (Dako, Carpinteria, CA) and a rabbit polyclonal anti-nitrotyrosine antibody (Life Technologies, Grand Island, NY). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was performed for DNA strand break assessment with the In Situ Cell Death Detection Kit, AP (Roche Diagnostics, Indianapolis, IN), as described in the manufacturer's instructions.
Du K., McGill M.R., Xie Y., Bajt M.L, & Jaeschke H. (2015). RESVERATROL PREVENTS PROTEIN NITRATION AND RELEASE OF ENDONUCLEASES FROM MITOCHONDRIA DURING ACETAMINOPHEN HEPATOTOXICITY. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 81, 62-70.
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2

Histopathological Assessment of Liver Necrosis

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Formalin-fixed liver samples were embedded in paraffin and 5 μm thick sections were cut. Sections were stained with hematoxylin and eosin (H&E) for evaluation of tissue necrosis (Gujral et al., 2002 (link)). Replicate sections were also stained for nitrotyrosine (NT) protein adducts for assessment of peroxynitrite formation using the Dako LSAB peroxidase kit (Dako, Carpinteria, CA) and a rabbit polyclonal anti-nitrotyrosine antibody (Life Technologies, Grand Island, NY) (Knight et al., 2002 (link)).
Du K., Williams C.D., McGill M.R, & Jaeschke H. (2014). Lower Susceptibility of Female Mice to Acetaminophen Hepatotoxicity: Role of Mitochondrial Glutathione, Oxidant Stress and c-Jun N-Terminal Kinase. Toxicology and applied pharmacology, 281(1), 58-66.
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3

Histological Assessment of Apoptosis and Necrosis in Liver Tissue

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Liver tissue samples embedded in paraffin were cut in 5 μm sections and stained with hematoxylin and eosin (H&E) for assessment of apoptosis versus necrosis (Gujral et al. 2002 (link)). Nitrotyrosine staining was performed as previously described (Knight et al. 2002 (link)), using a rabbit polyclonal anti-nitrotyrosine antibody (Life Technologies, Grand Island, NY) and the Dako LSAB peroxidase kit (Dako, Carpinteria, CA). Active caspase-3 staining was performed using a cleaved caspase-3 (Asp175) antibody (Cell Signaling Technology, Danvers, MA). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed for cell death using the In Situ Cell Death Detection Kit, AP (Roche Diagnostics, Indianapolis, IN) following manufacturer’s instructions.
Du K., Ramachandran A., Weemhoff J.L., Woolbright B.L., Jaeschke A.H., Chao X., Ding W.X, & Jaeschke H. (2018). Mito-Tempo Protects against Acute Liver Injury but Induces Limited Secondary Apoptosis during the Late Phase of Acetaminophen Hepatotoxicity. Archives of toxicology, 93(1), 163-178.
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