Goat anti mouse igg fitc antibody

Manufactured by Merck Group

Sourced in United States

The Goat anti-mouse-IgG-FITC antibody is a laboratory reagent used in various immunoassays and cell-based experiments. It is a polyclonal antibody produced in goats and conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate). The primary function of this antibody is to specifically bind to mouse immunoglobulin G (IgG) molecules, allowing for the detection and visualization of mouse IgG-containing samples or cells.

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Lab products found in correlation

Camedia c4040, by Olympus (1 mentions) Mouse monoclonal anti e cadherin antibody, by BD (1 mentions) B5002, by Merck Group (1 mentions) Rnase a, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Anti brdu antibody, by BD (1 mentions) Mouse anti γh2ax, by Merck Group (1 mentions) Rabbit anti 53bp1, by Cell Signaling Technology (1 mentions) H 1200, by Olympus (1 mentions) Rabbit igg f ab 2 fragment cy3, by Merck Group (1 mentions)

Close spelling variants of this product

Goat anti-mouse IgG-FITC secondary antibody FITC-conjugated goat anti-mouse IgG antibody Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG antibody FITC-conjugated goat anti-mouse IgG secondary antibody FITC)-labeled goat anti-mouse IgG antibody Goat anti-mouse IgG-FITC Anti-mouse IgG-FITC antibody Goat anti-mouse FITC Anti-mouse IgG-FITC FITC anti-mouse antibody

3 protocols using goat anti mouse igg fitc antibody

Immunofluorescence Staining of Epithelial and Mesenchymal Markers

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3 × 10⁴ cells of each of the cell lines were seeded on coverslips for 24 h in their respective media. Then, cells were fixed with 4% paraformaldehyde for 10 min, and permeabilized with 0.2% Triton X-100 in PBS for 20 min. Cells were blocked with blocking buffer (10% goat serum, 1% BSA, 0.2% triton X-100, and PBS 1X) for 1 h and then stained with the primary antibodies: mouse monoclonal anti-E-Cadherin antibody (Clone: 36/E-cadherin; BD Biosciences, San José, CA, USA), rabbit monoclonal anti-Vimentin antibody-Alexa Fluor-594 (Clone: EPR3776; Abcam, Cambridge, MA, USA), overnight at 4°C. After that, cells were incubated with the secondary antibody goat anti-mouse-IgG-FITC antibody (Sigma-Aldrich Co., St. Louis, MO, USA) for 30 min. Nuclei were stained with DAPI for 25 min. Cells were observed using a fluorescence microscope Olympus BX51, and images were acquired with a digital camera (Camedia C4040, Olympus).

Espinoza-Sánchez N.A., Chimal-Ramírez G.K., Mantilla A, & Fuentes-Pananá E.M. (2017). IL-1β, IL-8, and Matrix Metalloproteinases-1, -2, and -10 Are Enriched upon Monocyte–Breast Cancer Cell Cocultivation in a Matrigel-Based Three-Dimensional System. Frontiers in Immunology, 8, 205.

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Cell Cycle Analysis of CAPAN1 Cells

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Asynchronously growing CAPAN1 and CAPAN1.B2.S* cells were plated in 10 cm dishes (5x10⁵ cells/plate) and treated with 1 µM AZD-1775 or DMSO for 72 hours. The cells were pulse labelled with 30 µM BrdU (Sigma, B5002) for 1 hour prior to collection. Cells were harvested using trypsin, washed with PBS, fixed in cold 70% ethanol and stored at -20°C overnight. Samples were washed with 2 M NaCl/0.5% Triton X-100 and incubated for 30 minutes at room temperature. Cell pellets were resuspended in 0.1 M sodium tetraborate for 2 minutes and subsequent cell pellets were incubated at room temperature for 1 hour in anti-BrdU antibody (BD Biosciences, 347580) diluted in 0.5% TWEEN-20/1%BSA/PBS (1 µg antibody per 1 million cells). Sample pellets were washed in PBS/1% BSA. Cells were then incubated for 30 minutes at room temperature with goat anti-mouse IgG FITC antibody (Sigma, F0257) diluted in 0.5% TWEEN-20/1%BSA/PBS (1 µg antibody per 1 million cells). Cells were pelleted and resuspended in PBS containing 10 µg/ml RNaseA (Sigma, R4642) and 20 µg/ml propidium iodide (Sigma, P-4170) and incubated at room temperature for 30 minutes. Cell cycle analysis was performed on a FACS LSR II and analysed using FlowJo software (FlowJo, USA).

Dréan A., Williamson C.T., Brough R., Brandsma I., Menon M., Konde A., Garcia-Murillas I., Pemberton H.N., Frankum J., Rafiq R., Badham N., Campbell J., Gulati A., Turner N.C., Pettitt S.J., Ashworth A, & Lord C.J. (2017). Modelling therapy resistance in BRCA1/2 mutant cancers. Molecular cancer therapeutics, 16(9), 2022-2034.

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Immunofluorescent Assay for DNA Damage

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Cells grown on cover slips were fixed with 4% paraformaldehyde (PFA), and permeabilized in 0.3% Triton X-100. Cells were blocked at 37 °C for 30 min with 3% BSA in PBS supplemented with 3% donkey serum and 0.2% Triton X-100. Fixed cells were then incubated with diluted primary antibodies: Rabbit anti-53BP1 (Cell Signaling, 3428P), Mouse anti-γH2AX (Millipore, 05–636), Rabbit anti-NBS1-pSerine 343 (Epitomics, 2194–1-1), BRCA1-pS1524 (Bethyl, A300-001A), Mouse anti-TP53-pSerine 15 (Cell Signaling, 2524); Rabbit anti-RPA32-pSerine 33 (NOVUS, NB100–544), Rabbit anti-ssDNA (IBL,18731). After extensive wash by PBS, coverslips were incubated with secondary antibodies (Rabbit IgG F(ab′)₂ fragment-Cy3 and goat anti-Mouse IgG-FITC antibody, Sigma) for 30 min. After mounting with DAPI solution (VECTOR, H-1200), images were obtained using an Olympus epifluorescent microscope (BX 51) and analyzed with Image-pro plus (Applied Imaging). 100–200 cells or MN from 3 independent experiments were counted for quantitative immunofluorescent assays.

Tang Z., Yang J., Wang X., Zeng M., Wang J., Wang A., Zhao M., Guo L., Liu C., Li D, & Chen J. (2018). Active DNA end processing in micronuclei of ovarian cancer cells. BMC Cancer, 18, 426.

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