β lactoglobulin β lg from bovinemilk

Ovalbumin (Ova, ≥90%),
α-chymotrypsinogen A (α-CT) from bovine pancreas, myoglobin
(Myo) from equine skeletal muscle (≥95%), horseradish peroxidase
type VI-A (HRP), and β-lactoglobulin (β-LG) from bovine
milk (≥90%) were obtained from Sigma-Aldrich (Steinheim, Germany).
Appropriate amounts of protein powder were dissolved in SEC buffer.
Ultrapure water (MQ) was obtained with a Milli-Q system from Merck
Millipore (Darmstadt, Germany). Trifluoroacetic acid and acetonitrile,
both HPLC-grade, were purchased from AppliChem (Darmstadt, Germany).
All other chemicals used for the preparation of mobile phases were
obtained from Carl Roth (Karlsruhe, Germany).

Palm oil (refined, analytical standard grade), β-lactoglobulin (β-LG) from bovine milk (≧85%, lyophilized powder), and calcein were purchased from Sigma-Aldrich (St. Louis, MO, USA). SC (12.6–15.8% as N after drying), polyoxyethylene (20) sorbitan monooleate (Tween 80), SDS (>95%), and sodium chloride (NaCl) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Hexaglycerin monolaurate (HGML) and polyglycerin polycondensed ricinoleic acid ester (PGPR) were kindly donated by Nikko Chemicals Co., Ltd. (Tokyo, Japan). Skimmed milk powder containing 31.6 wt% casein protein and 5.3 wt% whey protein (as solid fraction) was obtained from Megmilk Snow Brand, Co., Ltd. (Tokyo, Japan). The water used in all experiments was prepared using a Direct-Q water purifier system (Merck Millipore Corporation, Billerica, MA, USA) and had 18.2 MΩ cm resistivity. All other chemicals were purchased from FUJIFILM Wako Pure Chemical Corporation and were of extra-pure grade. All chemicals were used as they were obtained without further purification.