Cd45 fitc 2d1

Manufactured by BD

CD45 FITC (2D1) is a fluorescent-labeled antibody that binds to the CD45 protein, which is expressed on the surface of various hematopoietic cells. The FITC (fluorescein isothiocyanate) fluorescent label is used to detect and quantify cells expressing CD45 in flow cytometry applications.

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Lab products found in correlation

Facscanto clinical flow cytometry system, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Cd4 v450 clone rpa t4, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Cd45ro pe cy7 uchl 1, by BD (1 mentions) Cd27 apc efluor780, by Thermo Fisher Scientific (1 mentions) Cd3 efluor450 okt3, by Thermo Fisher Scientific (1 mentions) Cd16 fitc 3g8, by BD (1 mentions) Pan γδtcr pe clone immu510, by Beckman Coulter (1 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Dm5000b, by Leica (1 mentions)

Spelling variants of this product

CD45-FITC (259 citations) CD45 (2D1, (6 citations) CD45 FITC (clone 2D1), (3 citations) Anti-human CD45-FITC (clone 2D1) (2 citations)

3 protocols using cd45 fitc 2d1

Characterizing hMSC Immunophenotype by Flow Cytometry

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The stability of the hMSCs’ immunophenotype was evaluated by flow cytometry. For these experiments, the cells were first washed with phosphate-buffered saline (PBS) and incubated with a non-specific blocking buffer containing 1% bovine serum albumin for 30 min. After centrifugation and removal of the blocking buffer, samples were treated with fluorescently conjugated mouse anti-human antibodies for 45 min. The expression of 5 surface markers was analyzed. The following antibodies were used: CD45 FITC (2D1), BD Biosciences; CD105 PE (TEA3/17.1.1), Beckman coulter; CD34 PerCP-Vio700 (AC136), MIltenyi Biotec; and CD90 APC (5E10), BioLegend. Vital dye -.DRAQ-7, BioLegend. Among the surface antigens detected, CD34 and CD45 are hematopoietic stem cell markers and thus are not expected to be expressed by MSCs while the others are MSC-specific markers [46 (link),47 (link)]. The stained cells were resuspended using PBS and analyzed in a BD FACSCanto™ Clinical Flow Cytometry System (BD Biosciences, San Jose, CA, USA).

Sindeeva O.A., Demina P.A., Kozyreva Z.V., Muslimov A.R., Gusliakova O.I., Laushkina V.O., Mordovina E.A., Tsyupka D., Epifanovskaya O.S., Sapach A.Y., Goryacheva I.Y, & Sukhorukov G.B. (2023). Labeling and Tracking of Individual Human Mesenchymal Stromal Cells Using Photoconvertible Fluorescent Microcapsules. International Journal of Molecular Sciences, 24(17), 13665.

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Flow Cytometry Antibody Panel

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Antibodies used for flow cytometry include: pan-γδTCR-PE (clone IMMU510; Beckman Coulter, Woerden, the Netherlands), pan-αβTCR-APC (clone IP26; eBioscience, Thermo Fisher Scientific), CD4-V450 (clone RPA-T4; BD Biosciences), CD8α-PerCP-Cy5.5 (RPA-T8; Biolegend), CD3-eFluor 450 (OKT-3; eBioscience), CD45-FITC (2D1; BD Biosciences), CD16-FITC (3G8; BD Biosciences), CD56-FITC (MY31; BD Biosciences), CD27-APC-eFluor780 (O323; eBioscience), CD45RO-PE-Cy7 (UCHL-1; BD Biosciences). All samples were analyzed on a BD LSRFortessa using FACSdiva software (BD Biosciences).

Straetemans T., Kierkels G.J., Doorn R., Jansen K., Heijhuurs S., dos Santos J.M., van Muyden A.D., Vie H., Clemenceau B., Raymakers R., de Witte M., Sebestyén Z, & Kuball J. (2018). GMP-Grade Manufacturing of T Cells Engineered to Express a Defined γδTCR. Frontiers in Immunology, 9, 1062.

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Immunophenotyping of Humanized Mice

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Sixteen weeks after CD34⁺ stem cell transplantation, reconstituted NSG mice were sacrificed and their spleens, small intestines and large intestines were harvested into ice cold RPMI containing 10% FBS. The intestines were flushed with ice cold PBS to remove fecal content and sliced into 1 cm pieces. Tissue samples were frozen in Tissue-Tek O.C.T compound (Sakura Finetek, Torrance, CA). Tissue sections of 6–8 μm were prepared on a cryotome and fixed on glass slides using cold 100% acetone for 10 minutes. After fixation, the sections were rehydrated in Tris-buffered saline (TBS) and blocked with TBS containing 20% w/v BSA and 0.1% v/v Tween-20. Sections were incubated with monoclonal antibodies targeting the following human antigens at room temperature for two hours: IgM-FITC (H15001), nucblue® fixed cell stain (both from Life Technologies, Grand Island, NY), CD3-PE (SK7), CD11c-APC (S-HCL-3), and CD45-FITC (2D1) all from (BD Pharmingen, San Diego, CA). Sections were subsequently washed with TBS containing 0.1% v/v Tween-20 and treated with Fluorogel (EMS, Hatfield, PA). The stained sections were analyzed using a fluorescence microscopy (Leica DM 5000B; Leica Microsystems, Wetzlar, Germany), and digital images were analyzed using LAS AF 1.8.1 software from Leica Microsystems.

Patton J., Vuyyuru R., Siglin A., Root M, & Manser T. (2015). Evaluation of the efficiency of human immune system reconstitution in NSG mice and NSG mice containing a human HLA.A2 transgene using hematopoietic stem cells purified from different sources. Journal of immunological methods, 422, 13-21.

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