Thioflavin t

Manufactured by BD

Thioflavin-T is a fluorescent dye used in the detection and quantification of amyloid fibrils. It exhibits a significant increase in fluorescence intensity upon binding to these protein aggregates.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Trucount, by BD (1 mentions) Lsrfortessa x 20 flow cytometer, by BD (1 mentions)

2 protocols using thioflavin t

Enumeration of Circulating and Disseminated Tumor Cells

Cited 1 time

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Enumeration of CTCs (by IE/FC and CellSearch) and DTCs was performed by
investigator JHS who was blinded to the study endpoints.
Blood and bone marrow samples were subjected to the IE/FC assay to
enumerate CTCs and DTCs, respectively (18 (link)–20 (link),22 (link)). Briefly, two distinct monoclonal antibodies
against EPCAM, one conjugated to immunomagnetic beads (MJ37) and the other
conjugated to phycoerythrin (EBA-1) were added to whole blood or bone marrow.
The sample was then placed in a magnet to capture cells labeled with the
magnetic bead-antibody conjugated. The supernatant containing cells that were
unbound (including red blood cells) was aspirated. Magnetic separation was
repeated twice to further enrich for EPCAM-expressing cells. A nucleic acid dye
(Thioflavin-T, BD Biosciences) and a monoclonal antibody to the
leukocyte-specific marker CD45 (2D1) conjugated to
peridinin-chlorophyll-protein-Cy5.5 were added to the sample. The enriched
sample was transferred to a BD TruCount™ (BD Biosciences) tube, and flow
cytometric analysis was performed using the BD FACSCalibur™ (BD
Biosciences). CTCs and DTCs were defined as nucleated cells that are
EPCAM-positive and CD45-negative.

Magbanua M.J., Yau C., Wolf D.M., Lee J.S., Chattopadhyay A., Scott J.H., Bowlby-Yoder E., Hwang E.S., Alvarado M., Ewing C.A., Delson A.L., van’t Veer L., Esserman L.J, & Park J.W. (2019). Synchronous detection of circulating tumor cells in blood and disseminated tumor cells in bone marrow predicts adverse outcome in early breast cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(17), 5388-5397.

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Thioflavin T Staining of C. albicans Infection

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iBMDM (2*10⁵ cells/well) were seeded in a 24-well plate overnight and then infected with C. albicans, or treated with LPS or thapsigargin for indicated timepoints. Thioflavin T (Cayman Chemical, 5 μM) was added 2 hours prior to endpoint. Cells were scraped into ice cold PBS and Thioflavin T intensity was measured on a BD LSRFortessa X-20 flow cytometer.

McFadden M.J., Reynolds M.B., Michmerhuizen B.C., Ólafsson E.B., Anderson F.M., Schultz T.L., O’Riordan M.X, & O’Meara T.R. (2024). Non-canonical activation of IRE1α during Candida albicans infection enhances macrophage fungicidal activity. bioRxiv.

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