Vero cells (# CCL-81), BHK-21 (# CCL-10), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), Rhabdomyosarcoma (RD) cells (# CCL-136), and MRC-5 (# CCL-171) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. Vero, BHK-21, MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, and CHO-pgsE-606 cells were grown in F-12K medium (Thermo Fisher # 21127022). MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03). Cells were cultured at 37 °C with 5% CO2. Cells were passaged at ~80 to 90% confluence and seeded as detailed for each individual assays. MucilAir™ (Epithelix # EP02MP) and SmallAir™ (Epithelix # EP21SA) HAE reconstituted from human primary cells obtained from nasal or bronchial biopsies were maintained in air–liquid interphase with specific culture medium (Epithelix # EP04MM, # EP64SA), in Costar Transwell inserts (Corning, NY, USA) per the manufacturer’s instructions.
Cho pgsa 745
Manufactured by Thermo Fisher Scientific
The CHO-pgsA-745 is a cell line derived from Chinese Hamster Ovary (CHO) cells. It is designed for the stable expression of recombinant proteins. The cell line expresses the pgsA gene, which is involved in the synthesis of proteoglycans.
Automatically generated - may contain errors
Lab products found in correlation
Cho k1, by American Type Culture Collection (4 mentions)
Bhk 21, by American Type Culture Collection (4 mentions)
Cho pgse 606, by American Type Culture Collection (4 mentions)
Cho pgsb 618, by American Type Culture Collection (4 mentions)
Bhk 21, by Thermo Fisher Scientific (4 mentions)
Cho pgsb 618, by Thermo Fisher Scientific (4 mentions)
Cho pgse 606, by Thermo Fisher Scientific (4 mentions)
Cho pgsa 745, by American Type Culture Collection (4 mentions)
F 12k medium, by Thermo Fisher Scientific (4 mentions)
Hek293ft, by American Type Culture Collection (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (4 mentions)
Vero cell, by American Type Culture Collection (4 mentions)
Glutamax, by Thermo Fisher Scientific (4 mentions)
Hek293ft, by Thermo Fisher Scientific (4 mentions)
Cho k1, by Thermo Fisher Scientific (4 mentions)
Monomac 1 cells, by Leibniz Institute DSMZ (4 mentions)
Mrc 5, by American Type Culture Collection (2 mentions)
Ep04mm, by Corning (2 mentions)
Costar transwell insert, by Corning (2 mentions)
Mrc 5, by Thermo Fisher Scientific (2 mentions)
4 protocols using cho pgsa 745
1
Characterization of Cell Lines and Airway Epithelia
2
Cell Culture Protocols for Diverse Cell Lines
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Vero cells (# CCL-81), BHK-21 (# CCL-10), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), Rhabdomyosarcoma (RD) cells (# CCL-136), and MRC-5 (# CCL-171) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. Vero, BHK-21, MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, and CHO-pgsE-606 cells were grown in F-12K medium (Thermo Fisher # 21127022). MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03). Cells were cultured at 37° C with 5% CO2. Cells were passaged at ~80–90% confluence and seeded as detailed for each individual assays. MucilAir™ (Epithelix # EP02MP) and SmallAir™ (Epithelix # EP21SA) HAE reconstituted from human primary cells obtained from nasal or bronchial biopsies were maintained in air-liquid interphase with specific culture medium (Epithelix # EP04MM, # EP64SA), in Costar Transwell inserts (Corning, NY, USA) per the manufacturer’s instructions.
3
Establishment of Cell Culture Protocols
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Vero cells (# CCL-81), BHK-21 (# CCL-10), Caco-2 (# HTB-37), Calu-3 (# HTB-55), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsD-677 (# CRL-2244), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), A549 (# CCL-185) and MOLT-4 (# CRL-1582) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. PBMCs were obtained from a healthy donor with informed consent, at the Department of Transfusion Medicine (NIH). Vero, BHK-21, Caco-2, Calu-3 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, CHO-pgsD-677, CHO-pgsE-606 and A549 cells were grown in F-12K medium (Thermo Fisher # 21127022). PBMCs, MOLT-4 and MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). BHK-21_hACE2, CHO-K1_hACE2, HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250–500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03), but Caco-2 with 20%, and cells were grown cultured at 37° C with 5% CO2 in sterile flasks. Cells were passaged at ~80–90% confluence and seeded as explained for each individual assays.
4
Establishment of Cell Culture Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Vero cells (# CCL-81), BHK-21 (# CCL-10), Caco-2 (# HTB-37), Calu-3 (# HTB-55), CHO-K1 (# CCL-61), CHO-pgsA-745 (# CRL-2242), CHO-pgsB-618 (# CRL-2241), CHO-pgsD-677 (# CRL-2244), CHO-pgsE-606 (# CRL-2246), HEK293-FT (# CRL-11268), A549 (# CCL-185) and MOLT-4 (# CRL-1582) cells were from the American Type Culture Collection (ATCC). MonoMac-1 cells (# ACC 252) were from the DSMZ-German Collection of Microorganisms and Cell Cultures. PBMCs were obtained from a healthy donor with informed consent, at the Department of Transfusion Medicine (NIH). Vero, BHK-21, Caco-2, Calu-3 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1, CHO-pgsA-745, CHO-pgsB-618, CHO-pgsD-677, CHO-pgsE-606 and A549 cells were grown in F-12K medium (Thermo Fisher # 21127022). PBMCs, MOLT-4 and MonoMac-1 cells were grown in RPMI 1640 (Thermo Fisher # 11875119). BHK-21_hACE2, CHO-K1_hACE2, HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250–500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v) not heat inactivated FBS (Hyclone # SH30071.03), but Caco-2 with 20%, and cells were grown cultured at 37° C with 5% CO2 in sterile flasks. Cells were passaged at ~80–90% confluence and seeded as explained for each individual assays.
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