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3 protocols using ammonium peroxy di sulfate aps

1

Graphite-based Photocatalyst Synthesis

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Pure graphite powder (99%;
LOBA Chemie, India, crystalline, 60 mesh), aniline (Fisher Scientific,
India), deionized water (Hydrolab, India), 5-sulfosalicylic acid (SSA,
Merck), ammonium peroxy-di-sulfate (APS, Merck), sodium nitrate (NaNO3, Merck), potassium permanganate (KMnO4, Merck),
sulfuric acid (H2SO4, Merck), ortho-phosphoric
acid (H3PO4, Merck), hydrogen peroxide (H2O2, Merck, 30% GR Pro analysis), calcium chloride
(CaCl2, Merck), phosphorus pentoxide (P2O5, Merck), and hydrazine hydrate (N2H4H2O, Merck) were used for the material synthesis. Malachite
green (MG), congo red (CR), and rhodamine B (RhB) dyes were purchased
from Himedia, India. Nitro B.T. AR (nitroblue tetrazolium (NBT)) and
benzene-1,4-dicarboxylic acid (teraphthalic acid (TA)) are procured
from LOBA Chemie, India.
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2

Electrochemical Sensing of Biomolecules

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Tyrase (EC 1.14.18.1; activity ≥ 2700 units mg−1 of solid, from mushroom), 3,4-dihydroxy-L-phenylalanine (L-DOPA), and 1,2-dihydroxybenzene (pyrocatechol) were acquired from Sigma Co., (Ronkonkoma, NY, USA). Multi-walled carbon nanotubes (MWCNTs) with outside dimensions 6–9 nm were purchased from Nutrino Company (Tehran, Iran). Corn starch was provided by Kimia Eksir Asia Company (Tehran, Iran). Aniline monomer, isopropanol, methanol, sodium hydroxide, chloroacetic acid, hydrogen chloride (HCl 37%), ammonium peroxy disulfate (APS), uric acid (UA), and ascorbic acid (AA) were acquired from Merck Company (Germany). All reagents were used without further purification. A phosphate buffer solution (PBS 0.1 M, pH = 6.8 at 25 ± 2 °C prepared by using K2HPO4 and KH2PO4) was used to prepare the electrolyte. All solutions were made ready with distilled water.
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3

Cellulase Enzyme Activity Assay

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Cellulase from Trichoderma reesei (Cat. No C2730, ≥700 U/g), bovine serum albumin (BSA), coomassie brilliant blue G-250, Whatman filter paper (Cat. No. 1001110), carboxymethyl cellulose (CMC), potassium sodium tartrate tetrahydrate, glucose, 3,5-dinitrosalicylic acid (DNS), aniline hydrochloride (99%, 𝑀𝑀 𝑤𝑤 =129.59 g/mol) and ammonium peroxydisulfate (APS, 98%, Mw =288.18 g/mol), were purchased from Merck AG (Germany). Poly(ε-caprolactone) (PCL, Mn=80000) and piperazine were obtained from Sigma Aldrich (United Kingdom). TVPO was synthesized according to a published work 24 (link) . Analytical grade phosphoric acid, ethanol, methanol, hydrochloric acid, acetone, chloroform, and dimethylformamide (DMF) were purchased from Merck (Germany).
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