Phalloidin conjugated to alexa flour 594

Triethylene glycol monomethyl ether (mTEG), 1,8-diazabicylo[5.4.0]undec-7-ene (DBU), 2,2′-azobis(2-methylpropionitrile) (AIBN), 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAT), ovotransferrin (OTF), 4-iodoanisole, and anhydrous dioxane were purchased from Sigma-Aldrich and used without further purification unless otherwise noted. The monomer 2-vinyl-4,4-dimethylazlactone (VDMA) was synthesized as previously described.⁶⁹ Fluorescein cadaverine (FC) was purchased from Biotium. Alexa Fluor 488 tetrafluorophenyl ester, NuPAGE 4–12% Bis-Tris gels, MES buffer, and LDS buffer were purchased from ThermoFisher Scientific. Inhibitor removal resin was purchased from Alfa Aesar. Holo-transferrin (HTf, Cat. No. 616397) was purchased from CalBiochem. PBS (10×) was purchased from Fisher Scientific. THF was purified using alumina drying columns. All other solvents were purchased from Pharmco-AAPER (Brookfield, CT). Deuterated DMSO (DMSO-d₆) and deuterated chloroform (CDCl₃) were purchased from Cambridge Isotope Laboratories, Inc. Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from ATCC, and all other cell culture reagents were obtained from Gibco. Phalloidin conjugated to Alexa Flour 594 was purchased from Thermo Fisher, formaldehyde as a 3.7% solution in PBS was from Fisher Scientific, and Vectashield mounting medium with DAPI was from Vector Labs.