To determine the elastic modulus of MeHA gels, mechanical measurements of hydrogels were performed using atomic force microscopy (AFM). Uncoated MeHA gels were fabricated by exposure to UV light as indicated and incubated in PBS for several hours before performing the AFM measurements. Mechanical measurements were performed on a JPK Nanowizard 4a AFM with a colloidal probe (sphere Ø = 3.5 μm) (CP-qp-CONT-SiO-B, NanoAndMore Corp., USA), a highly sensitive cantilever k = 0.1 N/m, and sample Poisson’s ratio of 0.5 at the UCLA Nano and Pico Characterization facility. During the measurement, hydrogels were incubated on a glass-bottom dish with prewarmed PBS and set on a temperature-controlled stage at 37°C. The force-distance curves were recorded, and the elastic modulus of hydrogels was calculated by NanoScope Analysis using the Hertz model.
Nanowizard 4a
Manufactured by Bruker
Sourced in United States
The Nanowizard 4a is an atomic force microscope (AFM) system designed for high-resolution imaging and characterization of nanoscale surfaces and structures. It provides precise control and measurement capabilities for a variety of applications in material science, life science, and nanotechnology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanowizard 4a
1
Elastic Modulus Determination of MeHA Hydrogels
2
Measuring Fibrin Gel Compressive Modulus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To analyze compressive modulus at a micro scale, 30 uL fibrin gels were fabricated as drops in 35-mm dishes and submerged in PBS. AFM indentation experiments were performed with a NanoWizard 4a (JPK Instruments) using a cantilever with a nominal spring constant of 0.03 N/m and a pyramidal tip (MLCT-D, Bruker AFM Probes). The dishes were maintained at 37°C during force measurements. Force curves were recorded from three separate regions on the surface of each hydrogel. At each region, 36 force curves were measured across a 100um2 area for a total of 108 measurements per gel. The data was analyzed using JPK image processing software. Young’s modulus was calculated using the Hertz model of fit on the extend curve, and data is represented as mean +/- standard deviation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!