Cdh13

Manufactured by Merck Group

Cdh13 is a lab equipment product offered by Merck Group. It functions as a cell adhesion protein, playing a role in cellular processes. The core function of Cdh13 is to facilitate cell-cell interactions and maintain tissue integrity.

Automatically generated - may contain errors

Lab products found in correlation

Biotinylated anti species secondary antibodies, by Vector Laboratories (1 mentions) Phosphohistone h 3 ph 3, by Merck Group (1 mentions) 5 bromodeoxyuridine brdu, by Progen Biotechnik (1 mentions) Cb 28, by Swant (1 mentions) Triton x 100, by Merck Group (1 mentions) Normal goat serum (ngs), by Merck Group (1 mentions) Cleaved caspase 3 cc3, by Cell Signaling Technology (1 mentions) Rmr622, by Biocare Medical (1 mentions) Diva decloaker, by Biocare Medical (1 mentions)

2 protocols using cdh13

Immunohistochemical Staining of Embryonic Brain Sections

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Embryonic brain sections were washed in PBS, blocked in a solution of 5% normal goat serum (Merck KGaA) (v/v) containing 0.1% Triton X-100 (v/v) (Merck KGaA) in PBS at RT for 2 h. They were first incubated in primary antibodies at RT for 2 h and, then, at 4 °C overnight. The following antibodies were used: mouse monoclonal Nestin (1:100, DSHB) and 5-Bromodeoxyuridine (BrdU; 1:1000, Progen), rat monoclonal anti-Ctip2 (1:500, Abcam), chicken polyclonal raised against GFP (1:500, Aves Laboratories), rabbit polyclonal raised against calbindin (CB-28; 1:3000, Swant), cleaved caspase-3 (CC3; 1:250, Cell Signalling Technology), Cux1 (1:100, Santa Cruz Biotechnology), Cdh13 (1:500, Millipore), L1 (L1; 1:1000, Millipore) or phospho-histone H-3 (PH-3; 1:1000, Millipore). Following incubation in primary antibodies, sections were washed in PBS, incubated in biotinylated anti-species secondary antibodies (1:250; Vector Laboratories) for 2 h and processed using conventional immunohistochemistry protocols described previously (Andrews et al. 2008 (link)).

Killen A.C., Barber M., Paulin J.J., Ranscht B., Parnavelas J.G, & Andrews W.D. (2017). Protective role of Cadherin 13 in interneuron development. Brain Structure & Function, 222(8), 3567-3585.

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Immunohistochemical Analysis of Aortic Tissue

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Formalin-fixed, paraffin embedded aortic tissue blocks were cut at 6μm thick. Slides were deparaffinized using Diva Decloaker (Biocare), blocked (Biocare, catalog No. RBM961) and stained for primary antibodies: Microsomal Glutathione S-Transferase 1 (MGST1; Abcam, catalog No. ab131059, 5.38μg/mL), ACTBL2 (ThermoFisher Scientific, catalog No. PA5-72402, 2μg/mL), CDH13 (Millipore, catalog No. abt121, 0.2μg/mL), LGALS9 (CellSignaling Technologies, catalog No. 55340, 0.27μg/mL), ORM2 (ThermoFisher Scientific, catalog No. PA5-37124, 5μg/mL), and PHB2 (CellSignaling Technology, catalog No. 14085, 1μg/mL). Secondary antibodies were used (Biocare, catalog No. RMR622) followed by 3,3′-Diaminobenzidine (DAB) chromogen and hematoxylin counterstain for nuclei. Slides were scanned and processed using QuPath (version 0.3.2, The University of Edinburgh) and images compiled in GraphPad Prism (version 8.0.0, San Diego, CA).

Tyrrell D.J., Chen J., Li B.Y., Wood S.C., Rosebury-Smith W., Remmer H.A., Jiang L., Zhang M., Salmon M., Ailawadi G., Yang B, & Goldstein D.R. (2022). Aging alters the aortic proteome in health and thoracic aortic aneurysm. Arteriosclerosis, thrombosis, and vascular biology, 42(8), 1060-1076.

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