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Omnic spectrum software

Manufactured by Thermo Fisher Scientific

OMNIC spectrum software is a data analysis tool that allows users to view, process, and analyze spectral data obtained from various analytical instruments, such as infrared (IR) and Raman spectrometers. The software provides a range of features to manipulate and interpret spectral information.

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2 protocols using omnic spectrum software

1

Infrared Spectroscopy of Sample Functional Groups

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Infrared spectra of the samples were analyzed to check the changes in the functional groups using Nicolet iZ10 (Thermo Fischer Scientific, Waltham, MA, USA) equipped with a golden gate diamond attenuated total reflectance (ATR) 10500 module with a germanium crystal using the single-reflection ATR method. Each spectrum was acquired by averaging 32 scans per sample in the wavelength range of 400–4000 cm−1 at 4 cm−1 resolutions and further analyzed using the OMNIC spectrum software (Thermo Fisher Scientific).
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2

FTIR Spectroscopic Analysis of Lyophilized Samples

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The infrared analysis was performed using FTIR spectroscopy (Thermo Scientific Nicolet iS20). The lyophilized sample was uniformly mixed with dry KBr at a weight ratio of 1:100. All the spectra were an average of 32 scans from 4000 to 400 cm−1 at a resolution of 4 cm−1 (Liu et al. 2019 (link)). The background noise was corrected with the data of pure KBr. The raw FTIR data were processed with Omnic spectrum software (Version 9.2.106; Thermo). Second derivative IR spectra were fitted simultaneously with the original IR spectra.
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